Conventional and Modern Bioassays-Detection, Isolation, Identification

Research output: Chapter in Book/Report/Conference proceedingChapter

13 Citations (Scopus)

Abstract

This chapter summarizes the principle and methodological solutions of bioactivity detection, which can be combined with planar layer chromatographic techniques. Antimicrobial, antioxidant, estrogenic, and various enzyme inhibition (acetyl- and butyryl-cholinesterase as well as α- and β-glucosidase) activity tests are described. The classification of antimicrobial assays, as well as the history of bioautography, is demonstrated with special emphasis on the advance of planar layer chromatography-bioassays. Direct bioautography (DB) and the advantage of the use of forced-flow planar layer chromatographic separation techniques are highlighted. Practice and applicability of DB including detection, quantification, as well as biomonitoring to control the isolation process are also included.

Original languageEnglish
Title of host publicationForced-Flow Layer Chromatography
PublisherElsevier Inc.
Pages347-395
Number of pages49
ISBN (Print)9780124201613
DOIs
Publication statusPublished - Jan 1 2016

Keywords

  • Bioluminescence
  • Direct bioautography
  • Effect-directed isolation
  • Hyphenations in planar layer chromatography
  • Planar layer chromatography-bioassays

ASJC Scopus subject areas

  • Chemistry(all)

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