Convenient enzymatic preparation of conjugated linoleic acid alkyl esters with C6-C22 alcohols

Csilla Dianóczki, Katalin Recseg, Katalin Kovári, László Poppe

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6 Citations (Scopus)


A fast and effective enzymatic process was developed for preparing CLA C6-C22 alkyl esters using immobilized Candida antarctica lipase B (Novozym 435). The reaction parameters (temperature, amount of starting materials and enzyme, effect of water removal during the reaction) were optimized. The re-use study showed that the enzyme can be used at least ten times without significant loss in its activity. Reactions between equimolar amounts of starting materials with 8.9% Novozym 435 (w/wCLA) at 65 °C in vacuum (20 mbar) for 1.5 h resulted in almost quantitatively (>95%) wax esters needing no further treatment. At room temperature, CLA esters with long chain (≥18 °C) saturated alcohols are solids.

Original languageEnglish
Pages (from-to)45-49
Number of pages5
JournalJournal of Molecular Catalysis B: Enzymatic
Issue number1-2
Publication statusPublished - Apr 1 2007



  • CLA ester
  • Conjugated linoleic acid ester
  • Esterification
  • Lipase
  • Solvent free condition

ASJC Scopus subject areas

  • Catalysis
  • Bioengineering
  • Biochemistry
  • Process Chemistry and Technology

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