Controversial results of the genetic analysis of a canine distemper vaccine strain

Zoltán Demeter, Elena Alina Palade, A. Hornyák, M. Rusvai

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Canine distemper (CD) is a highly contagious, often fatal, multisystemic viral disease of receptive carnivores. The presence of a PsiI cleavage site on a specific location of the hemagglutinin (H) gene was found to be a hallmark of vaccine strains, thus, a previously published restriction fragment length polymorphism (RFLP) test using PsiI theoretically allows the distinction between all currently used vaccine strains and virulent field strains. The RFLP test was carried out on all brands of CD vaccines available in Hungary. The present work describes the extensive sequencing and phylogenetic study of the strain present in Vanguard (Pfizer Animal Health) vaccines, which following the PsiI based RFLP test reacted as a wild-type strain. Based on the product description provided by the manufacturer, all batches should have contained a virus strain (Snyder Hill) belonging to the group of vaccine strains (America-1). Extensive genetic analysis involving the full nucleic acid sequence of four other genes (N, M, P and F) of the CDV genome revealed that the incriminated virus strain showed a higher level of genetic identity to wild-type strains from the America-2 group than to any of the strains belonging to America-1 group, therefore the vaccine does not contain the virus strain stated by the manufacturer in its product description and has not been containing it since at least 1992.

Original languageEnglish
Pages (from-to)420-426
Number of pages7
JournalVeterinary Microbiology
Volume142
Issue number3-4
DOIs
Publication statusPublished - May 2010

Fingerprint

Distemper
canine distemper
genetic techniques and protocols
Vaccines
vaccines
Restriction Fragment Length Polymorphisms
Viruses
restriction fragment length polymorphism
Hungary
Hemagglutinins
Virus Diseases
Pedigree
viruses
Nucleic Acids
Genes
Genome
testing
hemagglutinins
Health
carnivores

Keywords

  • Canine distemper
  • Phylogenetic analysis
  • Vaccine strain

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

Controversial results of the genetic analysis of a canine distemper vaccine strain. / Demeter, Zoltán; Palade, Elena Alina; Hornyák, A.; Rusvai, M.

In: Veterinary Microbiology, Vol. 142, No. 3-4, 05.2010, p. 420-426.

Research output: Contribution to journalArticle

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