Controlled reperfusion decreased reperfusion induced oxidative stress and evoked inflammatory response in experimental aortic-clamping animal model

G. Jancsó, E. Arató, P. Hardi, T. Nagy, Pintér, G. Fazekas, B. Gasz, I. Takacs, G. Menyhei, L. Kollar, L. Sínay

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Revascularization after long term aortic ischaemia in vascular surgery induces reperfusion injury accompanied with oxidative stress and inflammatory responses. The hypothesis of this study was that the aortic occlusion followed by controlled reperfusion (CR) can reduce the ischaemia-reperfusion injury, the systemic and local inflammatory response induced by oxidative stress. Animal model was used. Control group: animals underwent a 4-hour infrarenal aortic occlusion followed by continuous reperfusion. Treated group: animals were treated with CR: after a 4-hour infrarenal aortic occlusion we made CR for 30 minutes with the crystalloid reperfusion solution (blood: crystalloid solution ratio 1:1) on pressure 60 Hgmm. Blood samples were collected different times. The developing oxidative stress was detected by the plasma levels of malondialdehyde, reduced glutathion, thiol groups and superoxide dismutase. The inflammatory response was measured by phorbol myristate acetate-induced leukocyte reactive oxygen species production and detection of change in myeloperoxidase levels. The animals were anaesthetized one week after terminating ligation and biopsy was taken from quadriceps muscle and large parenchymal organs. CR significantly reduced the postischaemic oxydative stress and inflammatory responses in early reperfusion period. Pathophysiological results: The rate of affected muscle fibers by degeneration was significantly higher in the untreated animal group. The infiltration of leukocytes in muscle and parenchymal tissues was significantly lower in the treatedgroup. CR can improve outcome after acute lower-limb ischaemia. The results confirm that CR might be also a potential therapeutic approach in vascular surgery against reperfusion injury in acute limb ischaemia. Supported by OTKA K108596.

Original languageEnglish
Pages (from-to)217-234
Number of pages18
JournalClinical Hemorheology and Microcirculation
Volume63
Issue number3
DOIs
Publication statusPublished - 2016

Fingerprint

Constriction
Reperfusion
Oxidative Stress
Animal Models
Reperfusion Injury
Ischemia
Blood Vessels
Leukocytes
Muscles
Quadriceps Muscle
Tetradecanoylphorbol Acetate
Malondialdehyde
Sulfhydryl Compounds
Peroxidase
Superoxide Dismutase
Glutathione
Ligation
Lower Extremity
Reactive Oxygen Species
Extremities

Keywords

  • aortic ischaemia
  • Controlled reperfusion
  • reperfusion injury

ASJC Scopus subject areas

  • Physiology
  • Hematology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Controlled reperfusion decreased reperfusion induced oxidative stress and evoked inflammatory response in experimental aortic-clamping animal model. / Jancsó, G.; Arató, E.; Hardi, P.; Nagy, T.; Pintér; Fazekas, G.; Gasz, B.; Takacs, I.; Menyhei, G.; Kollar, L.; Sínay, L.

In: Clinical Hemorheology and Microcirculation, Vol. 63, No. 3, 2016, p. 217-234.

Research output: Contribution to journalArticle

Jancsó, G. ; Arató, E. ; Hardi, P. ; Nagy, T. ; Pintér ; Fazekas, G. ; Gasz, B. ; Takacs, I. ; Menyhei, G. ; Kollar, L. ; Sínay, L. / Controlled reperfusion decreased reperfusion induced oxidative stress and evoked inflammatory response in experimental aortic-clamping animal model. In: Clinical Hemorheology and Microcirculation. 2016 ; Vol. 63, No. 3. pp. 217-234.
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