Controlled ovarian stimulation and ultrasound guided follicular aspiration in the baboon (Papio cynocephalus anubis)

Sandor Cseh, Johannah Corselli, Philip Chan, Leonard Bailey

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The objective of this study was to investigate whether baboon females respond to an ovarian stimulation protocol incorporating pituitary suppression with a GnRH agonist (GnRHa) and highly purified human FSH (hphFSH) with follicular development and oocyte maturation. An ovulation induction protocol was applied to 5 adult female baboons with a history of regular menstrual cycles (33-34 days). A long-acting GnRHa implant containing goserelin acetate was placed s.c. on days 22-24 of their menstrual cycle. Daily hphFSH (75 IU im) treatments were started ∼ 10 days following menses. When the majority of the follicles were ≥ 5 mm in diameter and the E2 levels had reached a maximum, hCG (2000 IU i.m.) was administered to induce final maturation of the oocytes and ovulation. 30 to 34 h after hCG administration, transabdominal follicular aspiration was performed using a variable frequency transvaginal transducer with ultrasound. A total of 71 oocytes were collected (average: 17). 91% of the oocytes were morphologically normal indicating that they were appropriate for in vitro insemination.

Original languageEnglish
Pages (from-to)531-534
Number of pages4
JournalReproduction Nutrition Development
Volume41
Issue number6
DOIs
Publication statusPublished - 2001

Keywords

  • Baboon
  • GnRH agonist
  • Highly purified human FSH
  • Ovarian stimulation
  • Ultrasound-guided oocyte retrieval

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science
  • Embryology
  • Reproductive Medicine
  • Animal Science and Zoology
  • Developmental Biology

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