Controlled hydrostatic pressure stress downregulates the expression of ribosomal genes in preimplantation embryos: A possible protection mechanism?

I. Bock, H. Raveh-Amit, E. Losonczi, A. C. Carstea, A. Feher, K. Mashayekhi, S. Matyas, A. Dinnyés, C. Pribenszky

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The efficiency of various assisted reproductive techniques can be improved by preconditioning the gametes and embryos with sublethal hydrostatic pressure treatment. However, the underlying molecular mechanism responsible for this protective effect remains unknown and requires further investigation. Here, we studied the effect of optimised hydrostatic pressure treatment on the global gene expression of mouse oocytes after embryonic genome activation. Based on a gene expression microarray analysis, a significant effect of treatment was observed in 4-cell embryos derived from treated oocytes, revealing a transcriptional footprint of hydrostatic pressure-affected genes. Functional analysis identified numerous genes involved in protein synthesis that were downregulated in 4-cell embryos in response to hydrostatic pressure treatment, suggesting that regulation of translation has a major role in optimised hydrostatic pressure-induced stress tolerance. We present a comprehensive microarray analysis and further delineate a potential mechanism responsible for the protective effect of hydrostatic pressure treatment.

Original languageEnglish
Pages (from-to)776-784
Number of pages9
JournalReproduction, Fertility and Development
Volume28
Issue number6
DOIs
Publication statusPublished - 2016

Fingerprint

pressure treatment
Hydrostatic Pressure
Blastocyst
Down-Regulation
Gene Expression
protective effect
oocytes
genes
Embryonic Structures
Microarray Analysis
assisted reproductive technologies
gene expression
Oocytes
stress tolerance
translation (genetics)
Therapeutics
germ cells
Assisted Reproductive Techniques
protein synthesis
cells

Keywords

  • microarray
  • tolerance
  • transcription
  • translation.

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Reproductive Medicine
  • Developmental Biology
  • Endocrinology
  • Genetics
  • Molecular Biology
  • Biotechnology

Cite this

Controlled hydrostatic pressure stress downregulates the expression of ribosomal genes in preimplantation embryos : A possible protection mechanism? / Bock, I.; Raveh-Amit, H.; Losonczi, E.; Carstea, A. C.; Feher, A.; Mashayekhi, K.; Matyas, S.; Dinnyés, A.; Pribenszky, C.

In: Reproduction, Fertility and Development, Vol. 28, No. 6, 2016, p. 776-784.

Research output: Contribution to journalArticle

Bock, I. ; Raveh-Amit, H. ; Losonczi, E. ; Carstea, A. C. ; Feher, A. ; Mashayekhi, K. ; Matyas, S. ; Dinnyés, A. ; Pribenszky, C. / Controlled hydrostatic pressure stress downregulates the expression of ribosomal genes in preimplantation embryos : A possible protection mechanism?. In: Reproduction, Fertility and Development. 2016 ; Vol. 28, No. 6. pp. 776-784.
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