Control plant-parasitic nematodes with Trichoderma species and nematode-trapping fungi: The role of chi18-5 and chi18-12 genes in nematode egg-parasitism

Márton Szabó, Kitti Csepregi, Mónika Gálber, Ferenc Virányi, C. Fekete

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Eighteen strains of five Trichoderma species (Trichoderma atroviridae, Trichoderma harzianum, Trichoderma rossicum, Trichoderma tomentosum, Trichoderma virens) and six strains of four nematode-trapping fungi (Arthrobotrys oligospora, Arthrobotrys tortor, Monacrosporium haptotylum and Monacrosporium cionopagum) were tested in 90 combinations in order to identify the most appropriate partners to control plant-parasitic nematodes. In vitro monoculture growth rate tests, dual confrontation assays and comparison of strain specific egg-parasitic index (EPI) revealed that T. harzianum strains possess the strongest egg-parasitic ability and the best compatibility with M. cionopagum. Because Trichoderma chitinolytic enzyme systems play an important role in egg-parasitism the transcriptional activity of chi18-5 (chit42) and chi18-12 (chit33) of T. harzianum (SZMC 1647) during egg-parasitism were also characterized. The expression patterns of these genes revealed overlapping modes of action and elucidated differences in timing and amplitude of gene induction between the mycoparasitic and egg-parasitic processes.

Original languageEnglish
Pages (from-to)121-128
Number of pages8
JournalBiological Control
Volume63
Issue number2
DOIs
Publication statusPublished - Nov 2012

Fingerprint

nematophagous fungi
Trichoderma
plant parasitic nematodes
Trichoderma harzianum
parasitism
Nematoda
Trichoderma tomentosum
genes
Arthrobotrys
Arthrobotrys oligospora
Trichoderma virens
gene induction
mechanism of action
assays
enzymes
testing

Keywords

  • Caenorhabditis elegans model system
  • Chitinase gene-expression
  • Compatibility
  • Dual culture assays
  • Egg-parasitic index
  • In vitro growth rate tests

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Insect Science

Cite this

Control plant-parasitic nematodes with Trichoderma species and nematode-trapping fungi : The role of chi18-5 and chi18-12 genes in nematode egg-parasitism. / Szabó, Márton; Csepregi, Kitti; Gálber, Mónika; Virányi, Ferenc; Fekete, C.

In: Biological Control, Vol. 63, No. 2, 11.2012, p. 121-128.

Research output: Contribution to journalArticle

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