Control of the glutathione S-transferase and mas1′ promoter-driven GUS activity in auxin heterotrophic and autotrophic tobacco calli by exogenous 2,4-D-induced ethylene

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Abstract

Auxin autotrophic and heterotrophic lines of tobacco calli may differ not only in their indoleacetic acid (IAA) synthetizing abilities and sensitivities to exogenous auxins, but also in their gene expression patterns. Auxin autotrophic callus tissues from the leaf protoplasts of transgenic Nicotiana tabacum SR1 plants involving mas1′::GUS gene fusion were generated and the growth of cultures was compared with that of the heterotrophic lines of the same transgenic tissues on MS medium containing different concentrations of IAA or 2,4-D. The mas1′::GUS gene fusion expression was investigated, together with the glutathione S-transferase activities (GST, EC 2.5.1.18) in auxin autotrophic and heterotrophic tobacco calli. Both the mas1′ promoter and GST gene promoters contain ocs or ocs-like elements, responsible for both auxin and ethylene/wound inducibility. The mas1′ promoter exhibited a much higher expression activity in the heterotrophic cultures growing on IAA than in the autotrophic ones, but in contrast with the natural auxin, the mas1′::GUS activity decreased at elevated 2,4-D concentrations in the heterotrophic tissues and increased with increasing 2,4-D concentrations in the autotrophic lines. The induction of GST activity by different exogenous auxin concentrations was much higher in the autotrophic lines, especially in the case of 2,4-D. Higher concentrations of external 2,4-D resulted in increased ethylene production, which displayed different kinetics in the two types of calli. The ethylene-inducing 2,4-D concentrations increased the growth of the heterotrophic, but decreased that of the autotrophic lines. Blocking the ethylene receptors and hence the signal perception by 2,5-norbornadiene (NBD) in the heterotrophic tissues increased the 2,4-D-induced mas1′ promoter and GST activities, suggesting that the gaseous hormone counteracted the auxin response pathway. This was not found in the autotrophic tissues, where NBD decreased the mas1′-driven GUS activity. The GST activities were slightly decreased, or almost independent of the action of ethylene. It is suggested that the cross-talk between the auxin- and ethylene-induced signal transduction pathways may differ in the auxin autotrophic and heterotrophic lines.

Original languageEnglish
Pages (from-to)100-107
Number of pages8
JournalPhysiologia Plantarum
Volume113
Issue number1
DOIs
Publication statusPublished - 2001

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2,4-Dichlorophenoxyacetic Acid
Indoleacetic Acids
Bony Callus
Glutathione Transferase
glutathione transferase
2,4-D
Tobacco
ethylene
auxins
callus
tobacco
promoter regions
indole acetic acid
gene fusion
Gene Fusion
Heterotrophic Processes
genetically modified organisms
Gene Expression
Protoplasts
plant damage

ASJC Scopus subject areas

  • Plant Science

Cite this

@article{3673c3199a1347978a493e94c87663d8,
title = "Control of the glutathione S-transferase and mas1′ promoter-driven GUS activity in auxin heterotrophic and autotrophic tobacco calli by exogenous 2,4-D-induced ethylene",
abstract = "Auxin autotrophic and heterotrophic lines of tobacco calli may differ not only in their indoleacetic acid (IAA) synthetizing abilities and sensitivities to exogenous auxins, but also in their gene expression patterns. Auxin autotrophic callus tissues from the leaf protoplasts of transgenic Nicotiana tabacum SR1 plants involving mas1′::GUS gene fusion were generated and the growth of cultures was compared with that of the heterotrophic lines of the same transgenic tissues on MS medium containing different concentrations of IAA or 2,4-D. The mas1′::GUS gene fusion expression was investigated, together with the glutathione S-transferase activities (GST, EC 2.5.1.18) in auxin autotrophic and heterotrophic tobacco calli. Both the mas1′ promoter and GST gene promoters contain ocs or ocs-like elements, responsible for both auxin and ethylene/wound inducibility. The mas1′ promoter exhibited a much higher expression activity in the heterotrophic cultures growing on IAA than in the autotrophic ones, but in contrast with the natural auxin, the mas1′::GUS activity decreased at elevated 2,4-D concentrations in the heterotrophic tissues and increased with increasing 2,4-D concentrations in the autotrophic lines. The induction of GST activity by different exogenous auxin concentrations was much higher in the autotrophic lines, especially in the case of 2,4-D. Higher concentrations of external 2,4-D resulted in increased ethylene production, which displayed different kinetics in the two types of calli. The ethylene-inducing 2,4-D concentrations increased the growth of the heterotrophic, but decreased that of the autotrophic lines. Blocking the ethylene receptors and hence the signal perception by 2,5-norbornadiene (NBD) in the heterotrophic tissues increased the 2,4-D-induced mas1′ promoter and GST activities, suggesting that the gaseous hormone counteracted the auxin response pathway. This was not found in the autotrophic tissues, where NBD decreased the mas1′-driven GUS activity. The GST activities were slightly decreased, or almost independent of the action of ethylene. It is suggested that the cross-talk between the auxin- and ethylene-induced signal transduction pathways may differ in the auxin autotrophic and heterotrophic lines.",
author = "J. Csisz{\'a}r and M. Szab{\'o} and I. Tari and L. Erdei",
year = "2001",
doi = "10.1034/j.1399-3054.2001.1130114.x",
language = "English",
volume = "113",
pages = "100--107",
journal = "Physiologia Plantarum",
issn = "0031-9317",
publisher = "Wiley-Blackwell",
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TY - JOUR

T1 - Control of the glutathione S-transferase and mas1′ promoter-driven GUS activity in auxin heterotrophic and autotrophic tobacco calli by exogenous 2,4-D-induced ethylene

AU - Csiszár, J.

AU - Szabó, M.

AU - Tari, I.

AU - Erdei, L.

PY - 2001

Y1 - 2001

N2 - Auxin autotrophic and heterotrophic lines of tobacco calli may differ not only in their indoleacetic acid (IAA) synthetizing abilities and sensitivities to exogenous auxins, but also in their gene expression patterns. Auxin autotrophic callus tissues from the leaf protoplasts of transgenic Nicotiana tabacum SR1 plants involving mas1′::GUS gene fusion were generated and the growth of cultures was compared with that of the heterotrophic lines of the same transgenic tissues on MS medium containing different concentrations of IAA or 2,4-D. The mas1′::GUS gene fusion expression was investigated, together with the glutathione S-transferase activities (GST, EC 2.5.1.18) in auxin autotrophic and heterotrophic tobacco calli. Both the mas1′ promoter and GST gene promoters contain ocs or ocs-like elements, responsible for both auxin and ethylene/wound inducibility. The mas1′ promoter exhibited a much higher expression activity in the heterotrophic cultures growing on IAA than in the autotrophic ones, but in contrast with the natural auxin, the mas1′::GUS activity decreased at elevated 2,4-D concentrations in the heterotrophic tissues and increased with increasing 2,4-D concentrations in the autotrophic lines. The induction of GST activity by different exogenous auxin concentrations was much higher in the autotrophic lines, especially in the case of 2,4-D. Higher concentrations of external 2,4-D resulted in increased ethylene production, which displayed different kinetics in the two types of calli. The ethylene-inducing 2,4-D concentrations increased the growth of the heterotrophic, but decreased that of the autotrophic lines. Blocking the ethylene receptors and hence the signal perception by 2,5-norbornadiene (NBD) in the heterotrophic tissues increased the 2,4-D-induced mas1′ promoter and GST activities, suggesting that the gaseous hormone counteracted the auxin response pathway. This was not found in the autotrophic tissues, where NBD decreased the mas1′-driven GUS activity. The GST activities were slightly decreased, or almost independent of the action of ethylene. It is suggested that the cross-talk between the auxin- and ethylene-induced signal transduction pathways may differ in the auxin autotrophic and heterotrophic lines.

AB - Auxin autotrophic and heterotrophic lines of tobacco calli may differ not only in their indoleacetic acid (IAA) synthetizing abilities and sensitivities to exogenous auxins, but also in their gene expression patterns. Auxin autotrophic callus tissues from the leaf protoplasts of transgenic Nicotiana tabacum SR1 plants involving mas1′::GUS gene fusion were generated and the growth of cultures was compared with that of the heterotrophic lines of the same transgenic tissues on MS medium containing different concentrations of IAA or 2,4-D. The mas1′::GUS gene fusion expression was investigated, together with the glutathione S-transferase activities (GST, EC 2.5.1.18) in auxin autotrophic and heterotrophic tobacco calli. Both the mas1′ promoter and GST gene promoters contain ocs or ocs-like elements, responsible for both auxin and ethylene/wound inducibility. The mas1′ promoter exhibited a much higher expression activity in the heterotrophic cultures growing on IAA than in the autotrophic ones, but in contrast with the natural auxin, the mas1′::GUS activity decreased at elevated 2,4-D concentrations in the heterotrophic tissues and increased with increasing 2,4-D concentrations in the autotrophic lines. The induction of GST activity by different exogenous auxin concentrations was much higher in the autotrophic lines, especially in the case of 2,4-D. Higher concentrations of external 2,4-D resulted in increased ethylene production, which displayed different kinetics in the two types of calli. The ethylene-inducing 2,4-D concentrations increased the growth of the heterotrophic, but decreased that of the autotrophic lines. Blocking the ethylene receptors and hence the signal perception by 2,5-norbornadiene (NBD) in the heterotrophic tissues increased the 2,4-D-induced mas1′ promoter and GST activities, suggesting that the gaseous hormone counteracted the auxin response pathway. This was not found in the autotrophic tissues, where NBD decreased the mas1′-driven GUS activity. The GST activities were slightly decreased, or almost independent of the action of ethylene. It is suggested that the cross-talk between the auxin- and ethylene-induced signal transduction pathways may differ in the auxin autotrophic and heterotrophic lines.

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