Control of phosphatidylinositol turnover in adrenal glomerulosa cells

László Hunyady, Tamás Balla, Károly Nagy, András Spät

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The purpose of the present experiments was to compare the effects on phosphatidylinositol metabolism of agents stimulating aldosterone secretion. Glomerulosa cells, isolated from rat adrenals, were incubated in the presence of one of the following stimuli: angiotensin II, elevated potassium concentration, corticotropin, dibutyryl cyclic AMP and prostaglandin E2. Of all these substances, only angiotensin II stimulated the incorporation of [32Piphosphate into phosphatidylinositol. The effect was already detected 2.5 min and was still maintained 60 min after the onset of stimulation. A slight enhancement of the incorporation into other phospholipids was observed in the first minutes of stimulation. Cycloheximide abolished the effect of angiotensin II on aldosterone production, but not on phosphatidylinositol synthesis. In cells prelabelled with [32P]phosphate, radioactivity in phosphatidylinositol relative to that in other phospholipids decreased in response to angiotensin II within 5 min. This indicates that angiotensin II induces a specific breakdown of phosphatidylinositol. Corticotropin failed to enhance the incorporation of [32P]phosphate into phosphatidylinositol and other phospholipids in isolated fasciculate-reticularis cells. The results suggests that although both angiotensin II and potassium are presumed to act through changes in calcium metabolism, angiotensin alone generates the calcium signal by increased phosphatidylinositol turnover.

Original languageEnglish
Pages (from-to)352-357
Number of pages6
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume713
Issue number2
DOIs
Publication statusPublished - Nov 12 1982

Keywords

  • (Adrenal glomerulosa cell)
  • ACTH
  • Aldosterone synthesis
  • Angiotensin II
  • K
  • Phosphatidylinositol
  • Phospholipid turnover

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Endocrinology

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