Control of late off-center cone bipolar cell differentiation and visual signaling by the homeobox gene Vsx1

Robert L. Chow, B. Völgyi, Rachel K. Szilard, David Ng, Colin McKerlie, Stewart A. Bloomfield, David G. Birch, Roderick R. McInnes

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

Retinal bipolar cells are interneurons that transmit visual signals from photoreceptors to ganglion cells. Although the visual pathways mediated by bipolar cells have been well characterized, the genes that regulate their development and function are largely unknown. To determine the role in bipolar cell development of the homeobox gene Vsx1, whose retinal expression is restricted to a major subset of differentiating and mature cone bipolar (CB) cells, we targeted the gene in mice. Bipolar cell fate was not altered in the absence of Vsx1 function, because the pan-bipolar markers Chx10 and Ret-B1 continued to be expressed in inner nuclear layer neurons labeled by the Vsx1-targeting reporter gene, τLacZ. The specification, number, and gross morphology of the subset of on-center and off-center (OFF)-CB cells defined by τLacZ expression from the Vsx1 locus were also normal in Vsx1 τLacZ/Vsx1τLacZ mice. However, the terminal differentiation of OFF-CB cells in the retina of Vsx1τLacZ/ Vsx1τLacZ mice was incomplete, as demonstrated by a substantial reduction in the expression of at least four markers (recoverin, NK3R, Neto1, and CaB5) for these interneurons. These molecular abnormalities were associated with defects in retinal function and documented by electroretinography and in vitro ganglion cell recordings specific to cone visual signaling. In particular, there was a general reduction in the light-mediated activity of OFF, but not on-center, ganglion cells. Thus, Vsx1 is required for the late differentiation and function of OFF-CB cells and is associated with a heritable OFF visual pathway-specific retinal defect.

Original languageEnglish
Pages (from-to)1754-1759
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number6
DOIs
Publication statusPublished - Feb 10 2004

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Homeobox Genes
Cell Differentiation
Ganglia
Visual Pathways
Interneurons
Retinal Bipolar Cells
Recoverin
Electroretinography
Reporter Genes
Genes
Retina
Neurons
Light

ASJC Scopus subject areas

  • Genetics
  • General

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Control of late off-center cone bipolar cell differentiation and visual signaling by the homeobox gene Vsx1. / Chow, Robert L.; Völgyi, B.; Szilard, Rachel K.; Ng, David; McKerlie, Colin; Bloomfield, Stewart A.; Birch, David G.; McInnes, Roderick R.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, No. 6, 10.02.2004, p. 1754-1759.

Research output: Contribution to journalArticle

Chow, Robert L. ; Völgyi, B. ; Szilard, Rachel K. ; Ng, David ; McKerlie, Colin ; Bloomfield, Stewart A. ; Birch, David G. ; McInnes, Roderick R. / Control of late off-center cone bipolar cell differentiation and visual signaling by the homeobox gene Vsx1. In: Proceedings of the National Academy of Sciences of the United States of America. 2004 ; Vol. 101, No. 6. pp. 1754-1759.
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