In cultured glial cells from chick embryonic brain, both influx and efflux of 42K+ and 22Na+ are dependent on the external Ca++ concentration ([Ca++]0) between 2 and 0.1 mM although intracellular concentrations of K+ ([K+](i)) and Na+ ([Na+](i)) do not change. Only a reduction of [Ca++]0 below 0.1 mM results in both a decrease of [K+](i) and an increase of [Na+](i). Ouabain significantly decreases the [Ca++]0 sensitivity of uphill cation movements (K+ influx and Na+ efflux), while the [Ca++]0 sensitivity of downhill cation movements (K+ efflux and Na+ influx) is almost unaffected by the presence of ouabain. Additionally, a decrease in [Ca++]0 triggers an increase in intracellular concentration of adenosine 3':5'-cyclic monophosphoric acid (cAMP). These findings suggest that changes of [Ca++]0, which take place in vivo in the microenvironment of the glia after neuronal firing, represent a signal in the glial-neuronal interaction controlling cation transport and that this control is achieved by a co-operation between the cAMP-generating and the cation transport system.
ASJC Scopus subject areas
- Developmental Neuroscience