Contribution of porcine follicular fluid in the process of fertilization in vivo

K. P. Brüssow, J. Rátky, H. Torner, P. Sarlós, L. Solti

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8 Citations (Scopus)

Abstract

Two experiments were conducted to determine if follicular fluid (FF) enters the oviduct and plays any role in the postovulatory distribution of sperm cells within the oviduct. Oestrus and ovulation were synchronized by feeding of Regumate® and application of 1,000 IU PMSG and 500 IU hCG. Experiment I: The steroid contents of FF in relation to the oviductual fluid prior to ovulation and within the oviductual fluid before and after ovulation were analyzed in group 1 (n=7). For this purpose aspiration of FF from follicles and salpingectomy of the left ovary was laparoscopically performed prior to ovulation (34-36 h after hCG). The oviduct of the right control side was removed after ovulation (42-44 h after hCG). To prevent the entry of FF into the oviduct, follicles of the left ovary were aspirated (group 2, n=7) or the left oviduct was ligated between ampulla and infundibulum (group 3, n=7) prior to ovulation. Bilateral salpingectomy were done after ovulation, respectively. Oviducts were flushed with 1 ml saline and the samples were assayed for steroids by RIA methods. In group 1 the progesterone concentration within the oviductual flushing did not differ before and after ovulation (0.09±0.13 vs. 0.12±0.16ng ml-1). Withdrawal of FF from the left ovary by aspiration (group 2) or ligation of the oviduct (group 3) did not influence the steroid content within the oviducts. Similar low concentrations were measured in left and right oviducts after ovulation (0.29±0.17 and 0.22±0.19 vs. 0.24±0.35 and 0.21± 0.22 ng ml-1). The high content of follicular fluid progesterone (269.7±67.9 and 389.6±226.5 ng ml-1 in group 1 and 2, respectively) was not reflected in oviductual fluid. Compared to FF, only 0.03 to 0.07 % of progesterone concentration were found within the oviductuad flushings. Experiment II: Gilts were inseminated time-oriented with fresh semen (4×109 sperm cells/ 100 ml) 36 h after hCG injection. To prevent the entry of FF into the oviduct unilaterally the left oviduct was ligated between the ampulla and infundibulum (group 1, n=18), FF of follicles of the left ovary was aspirated (group 2, n=8) or the infundibulum was taken from the ovary and fixed in an adovarian position at the uterine wall and sutured with endoscopical knots (group 3, n=8). The right oviduct served as control. All endoscopic handling was done 30 minutes after insemination. Bilateral salpingectomy was performed postovulatory (56.5 h after hCG, i.e. 20.5 h post insemination, respectively) by laparotomy. The ampullary and isthmic sections of the oviducts were flushed separately with 0.25 ml PBS. Ligation of the oviduct (n=10 gilts) seems to influence sperm cell distribution within the oviductal sections (0 and 12.4×103 sperm cells in the ligated ampullary and isthmic sections vs. 14.6 and 42.9×103 in the control). But it remained open whether the different sperm cell distribution is related to the failure of FF or to the surgical ligation procedure. Therefore, additionally in 24 gilts a ligation, shame-ligation or the FF withdrawal by aspiration was carried out. Manipulation of the left oviduct resulted in a reduced number of sperm cells compared to the contralateral control oviduct (ligation - 5.47, aspiration - 10.67, shame-ligation 9.89×103 sperm cells vs. 16.14, 14.05 and 18.21×103 sperm cells in the right control oviducts, respectively). However due to the high variation in sperm cell number these differences are not significant. Altogether we conclude that 1) only an unimportant amount of FF reaches the oviduct at ovulation and 2) FF is not a trigger of sperm cell distribution within the porcine oviduct at ovulation.

Original languageEnglish
Pages (from-to)139-145
Number of pages7
JournalReproduction in Domestic Animals
Volume34
Issue number3-4
DOIs
Publication statusPublished - Jan 1 1999

ASJC Scopus subject areas

  • Biotechnology
  • Animal Science and Zoology
  • Endocrinology

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