Contribution of phopholipase D and a brefeldin A-sensitive ARF to chemoattractant-induced superoxide production and secretion of human neutrophils

Krisztina Káldi, Júlia Szeberényi, Balázs K. Rada, Péter Kovács, Miklós Geiszt, Attila Mócsai, Erzsébet Ligeti

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We show that blockers of phospholipase D (PLD) reduce fMLP-triggered exocytosis of secretory vesicles effectively. In accordance with this, the PLD product phosphatidic acid (PA) was able to induce mobilization of secretory vesicles. Although PLD seems to play a role in the release of all neutrophil granule types, exogenous PA alone was not sufficient to activate the exocytosis of primary and secondary granules, suggesting that in the case of these granules, additional signaling factors are required to initiate the secretory responses. The ADP-ribosylation factor (ARF)-inhibitor brefeldin A (BFA) inhibited the fMLP-stimulated O2.- production strongly, whereas it did not influence any of the exocytic responses, and no significant effect of BFA was detected on the O2.- generation induced by other stimuli. On the basis of these results, we propose that upon chemoattractant stimulation, PLD activity is involved in induction of degranulation and O2.- production, but a BFA-sensitive ARF is ouly required to the activation of the NADPH oxidase. This ARF action seems to participate exclusively in the signaling pathway between the fMLP receptor and the oxidase.

Original languageEnglish
Pages (from-to)695-700
Number of pages6
JournalJournal of Leukocyte Biology
Volume71
Issue number4
Publication statusPublished - Apr 1 2002

Keywords

  • NADPH oxidase
  • Phagocytes
  • Secretory vesicles
  • fMLP receptor

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology

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