Contribution of membrane lipids to the ability of the photosynthetic machinery to tolerate temperature stress

Hajime Wada, Zoltan Gombos, Norio Murata

Research output: Contribution to journalArticle

137 Citations (Scopus)

Abstract

The contribution of the unsaturation of membrane lipids to the ability of the photosynthetic machinery to tolerate temperature stress was studied in a transgenic cyanobacterium. Anacystis nidulans R2-SPc was transformed with the desA gene, which encodes the Δ12-desaturase that desaturates the fatty acids of membrane lipids in Synechocystis PCC6803. The transformant acquired the ability to introduce a second double bond into palmitoleic and oleic acids. The transformation enhanced the tolerance of the photosynthetic machinery to chilling stress but it had no detectable effect on the ability to tolerate heat stress. The transformation itself did not have any effect on photosynthetic activity. These results imply that an increase in the unsaturation of membrane lipids enhances the tolerance of the photosynthetic machinery toward chilling stress but not toward heat stress and that such an increase does not affect photosynthesis within the range of physiological temperatures.

Original languageEnglish
Pages (from-to)4273-4277
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number10
Publication statusPublished - May 10 1994

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Membrane Lipids
Temperature
Hot Temperature
Fatty Acid Desaturases
Oleic Acids
Synechocystis
Photosynthesis
Cyanobacteria
Genes

Keywords

  • cyanobacteria
  • desA gene
  • desaturation
  • fatty acid
  • transformation

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

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AB - The contribution of the unsaturation of membrane lipids to the ability of the photosynthetic machinery to tolerate temperature stress was studied in a transgenic cyanobacterium. Anacystis nidulans R2-SPc was transformed with the desA gene, which encodes the Δ12-desaturase that desaturates the fatty acids of membrane lipids in Synechocystis PCC6803. The transformant acquired the ability to introduce a second double bond into palmitoleic and oleic acids. The transformation enhanced the tolerance of the photosynthetic machinery to chilling stress but it had no detectable effect on the ability to tolerate heat stress. The transformation itself did not have any effect on photosynthetic activity. These results imply that an increase in the unsaturation of membrane lipids enhances the tolerance of the photosynthetic machinery toward chilling stress but not toward heat stress and that such an increase does not affect photosynthesis within the range of physiological temperatures.

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