Contribution of individual flavonoids in Lysimachia species to the antioxidant capacity based on HPLC-DPPH assay

Anita Toth, Eszter Riethmuller, Krisztina Vegh, Agnes Alberti, S. Béni, A. Kéry

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Quantitative phytochemical characterisation of the chief flavonoid aglycones in the hydrolysed Lysimachia extracts revealed the dominance of kaempferol, quercetin and myricetin in L. vulgaris, L. nummularia, L. punctata, L. christinae, L. ciliata and L. clethroides, respectively. Due to the significant radical scavenging capacity of the samples, the contribution of the individual aglycones to the total antioxidant activity became of interest. Therefore, a HPLC method coupled to pre-column DPPH scavenging assay was developed. Differences in the six Lysimachia species’ phenolic composition regarding their participation to the antioxidant activity were revealed. The participation of the three investigated flavonoids to the radical quenching activity was the highest (91.2%) in the L. vulgaris sample, the lowest in L. christinae sample with 29.6%. In L. vulgaris sample, the 76.3% contribution of quercetin to the scavenger capacity was the highest peak area decrement ratio among the investigated samples.

Original languageEnglish
Pages (from-to)1-4
Number of pages4
JournalNatural Product Research
DOIs
Publication statusAccepted/In press - Jul 28 2017

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Keywords

  • antioxidant
  • flavonoid
  • HPLC-DPPH
  • Lysimachia

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Plant Science
  • Organic Chemistry

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