Contrasting effects of RNA and protein synthesis blocking on natural and lectin-dependent cell-mediated cytotoxicity against adherent HEp-2 cells

A. Perl, R. Gonzalez-Cabello, L. Falucskai, P. Gergely, J. Fehér

Research output: Contribution to journalArticle

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Abstract

In this study, earlier observations2,9 concerning the independence of both natural (NCMC) and lectin-dependent cell-mediated cytotoxicity (LDCC) from DNA synthesis have been confirmed. In addition, blocking of RNA synthesis by actinomycin D and of protein synthesis, reversibly by puromycin (PM) and irreversibly by emetine (EM) had different effects on NCMC and LDCC against3H-thymidine-prelabeled HEp-2 target cells. Similarly to the Con A-induced proliferation of lymphocytes, LDCC activity was also inhibited by blocking of RNA and protein synthesis. NCMC to HEp-2 target cells was not affected by blocking of RNA synthesis, while both PM and EM strongly enhanced NCMC activity.

Original languageEnglish
Pages (from-to)1344-1346
Number of pages3
JournalExperientia
Volume41
Issue number10
DOIs
Publication statusPublished - Oct 1985

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Cytotoxicity
Lectins
RNA
Emetine
Puromycin
Proteins
Lymphocytes
Dactinomycin
Thymidine
DNA

Keywords

  • lectin-dependent cell-mediated cytotoxicity
  • Natural cell-mediated cytotoxicity
  • protein synthesis
  • RNA synthesis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Contrasting effects of RNA and protein synthesis blocking on natural and lectin-dependent cell-mediated cytotoxicity against adherent HEp-2 cells. / Perl, A.; Gonzalez-Cabello, R.; Falucskai, L.; Gergely, P.; Fehér, J.

In: Experientia, Vol. 41, No. 10, 10.1985, p. 1344-1346.

Research output: Contribution to journalArticle

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