Consumption of conjugated linoleic acid (CLA)-supplemented diet during colitis development ameliorates gut inflammation without causing steatosis in mice

Thais Garcias Moreira, Ana Cristina Gomes-Santos, Laila Sampaio Horta, Mariana Camila Goncalves, Andrezza Fernanda Santiago, Juliana Gonçalves Lauar, Daniela Silva dos Reis, Archimedes Barbosa Castro-Junior, Luisa Lemos, Mauro Guimarães, Edenil Costa Aguilar, Attila Pap, Joana Ferreira Amaral, Jacqueline I. Alvarez-Leite, Denise Carmona Cara, Rafael Machado Rezende, Laszlo Nagy, Ana Maria Caetano Faria, Tatiani Uceli Maioli

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1 Citation (Scopus)

Abstract

Dietary supplementation with conjugated linoleic acid (CLA) has been proposed for weight management and to prevent gut inflammation. However, some animal studies suggest that supplementation with CLA leads to the development of nonalcoholic fatty liver disease. The aims of this study were to test the efficiency of CLA in preventing dextran sulfate sodium (DSS)-induced colitis, to analyze the effects of CLA in the liver function, and to access putative liver alterations upon CLA supplementation during colitis. So, C57BL/6 mice were supplemented for 3 weeks with either control diet (AIN-G) or 1% CLA-supplemented diet. CLA content in the diet and in the liver of mice fed CLA containing diet were accessed by gas chromatography. On the first day of the third week of dietary treatment, mice received ad libitum a 1.5%–2.5% DSS solution for 7 days. Disease activity index score was evaluated; colon and liver samples were stained by hematoxylin and eosin for histopathology analysis and lamina propria cells were extracted to access the profile of innate cell infiltrate. Metabolic alterations before and after colitis induction were accessed by an open calorimetric circuit. Serum glucose, cholesterol, triglycerides and alanine aminotransaminase were measured; the content of fat in liver and feces was also accessed. CLA prevented weight loss, histopathologic and macroscopic signs of colitis, and inflammatory infiltration. Mice fed CLA-supplemented without colitis induction diet developed steatosis, which was prevented in mice with colitis probably due to the higher lipid consumption as energy during gut inflammation. This result suggests that CLA is safe for use during gut inflammation but not at steady-state conditions.

Original languageEnglish
Pages (from-to)238-245
Number of pages8
JournalJournal of Nutritional Biochemistry
Volume57
DOIs
Publication statusPublished - Jul 2018

Keywords

  • Colitis
  • Conjugated Linoleic Acid
  • DSS
  • Inflammation
  • Metabolism
  • Steatosis

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology
  • Nutrition and Dietetics
  • Clinical Biochemistry

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  • Cite this

    Moreira, T. G., Gomes-Santos, A. C., Horta, L. S., Goncalves, M. C., Santiago, A. F., Lauar, J. G., dos Reis, D. S., Castro-Junior, A. B., Lemos, L., Guimarães, M., Aguilar, E. C., Pap, A., Amaral, J. F., Alvarez-Leite, J. I., Cara, D. C., Rezende, R. M., Nagy, L., Faria, A. M. C., & Maioli, T. U. (2018). Consumption of conjugated linoleic acid (CLA)-supplemented diet during colitis development ameliorates gut inflammation without causing steatosis in mice. Journal of Nutritional Biochemistry, 57, 238-245. https://doi.org/10.1016/j.jnutbio.2018.04.003