Construction of super-resolution microscope based on cw laser light source

Yoshinori Iketaki, Takeshi Watanabe, N. Bokor, Masaaki Fujii

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We constructed a super-resolution microscope system based on a cw laser light source. Electro-optical modulators convert the cw laser light into a light pulse with a width of 15 ns and provide a repetition rate of 100 kHz. The performance of the microscope was evaluated using 100 nm φ fluorescence microbeads. The fluorescence signal from the beads can be detected with very low statistical fluctuation. The super-resolution image was obtained with better signal to noise ratio compared with that given by a pulse laser light source with a repetition rate of several tens of hertz. The fluorescent size of the beads was 150 nm, which is 2.3 times smaller than the diffraction limit. Experimental results confirmed that the two-point resolution also overcomes the diffraction limit. The constructed system is expected to become a commercial microscope.

Original languageEnglish
Article number063112
JournalReview of Scientific Instruments
Volume77
Issue number6
DOIs
Publication statusPublished - 2006

Fingerprint

Light sources
light sources
Microscopes
microscopes
beads
Lasers
Laser pulses
repetition
Diffraction
Fluorescence
lasers
fluorescence
Light modulators
image resolution
Image resolution
pulses
diffraction
modulators
Signal to noise ratio
signal to noise ratios

ASJC Scopus subject areas

  • Instrumentation
  • Physics and Astronomy (miscellaneous)

Cite this

Construction of super-resolution microscope based on cw laser light source. / Iketaki, Yoshinori; Watanabe, Takeshi; Bokor, N.; Fujii, Masaaki.

In: Review of Scientific Instruments, Vol. 77, No. 6, 063112, 2006.

Research output: Contribution to journalArticle

Iketaki, Yoshinori ; Watanabe, Takeshi ; Bokor, N. ; Fujii, Masaaki. / Construction of super-resolution microscope based on cw laser light source. In: Review of Scientific Instruments. 2006 ; Vol. 77, No. 6.
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