Construction of a recombinant herpesvirus expressing the jellyfish green fluorescent protein

Z. Boldogkői, F. Erdélyi, Attila Sik, T. Freund, István Fodor

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Here we report the insertion of a synthetic version of the cDNA encoding the jellyfish (Aequorea victoria) green fluorescent protein (gfph) into the genome of pseudorabies (Aujeszky's disease) virus (PrV). A putative latency promoter (PLAT) located at the inverted repeat region of the PrV genome was chosen as the target site for the insertion. Recombinant viral DNA designated as vLAT-gfp was generated as a result of homologous recombination between the transfected viral DNA and a plasmid containing the GFP-expression cassette flanked by viral sequences homologous to the target region. Plaques containing recombinant virus were selected visually using a fluorescent microscope. We demonstrated a GFP-expression in infected neurons of rat brain which showed normal morphology at early stage of viral infection by monitoring fluorescent light emission.

Original languageEnglish
Pages (from-to)69-74
Number of pages6
JournalLuminescence
Volume14
Issue number2
Publication statusPublished - Mar 1999

Fingerprint

Herpesviridae
Viral DNA
Green Fluorescent Proteins
Viruses
Genes
Pseudorabies
Genome
Suid Herpesvirus 1
Recombinant DNA
Homologous Recombination
Light emission
Virus Diseases
Sequence Homology
Neurons
Rats
Brain
Microscopes
Plasmids
Complementary DNA
Light

Keywords

  • Gene expression
  • Green fluorescent protein
  • Pseudorabies (Aujeszky's disease) virus

ASJC Scopus subject areas

  • Biophysics

Cite this

Construction of a recombinant herpesvirus expressing the jellyfish green fluorescent protein. / Boldogkői, Z.; Erdélyi, F.; Sik, Attila; Freund, T.; Fodor, István.

In: Luminescence, Vol. 14, No. 2, 03.1999, p. 69-74.

Research output: Contribution to journalArticle

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