Construction and analysis of 2 reciprocal arabidopsis introgression line populations

O. Törjék, Rhonda C. Meyer, Maik Zehnsdorf, Melanie Teltow, Georg Strompen, Hanna Witucka-Wall, Anna Blacha, Thomas Altmann

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Two new large reciprocal sets of introgression lines (ILs) were created between the Arabidopsis accessions Col-0 and C24. In both sets (78 ILs with Col-0 background and 62 ILs with C24 background), the donor segments cover almost the entire genome with an average substitution size of 18.3 cM. In addition to the basic sets of ILs, further subILs were developed for 2 genomic regions allowing better mapping resolution. SubILs carrying donor segments with candidate genes for flowering time and reduced fertility were used to demonstrate the usefulness of the reciprocal ILs for quantitative trait loci detection and fine mapping. For subIL development at high resolution around the reduced fertility locus, we used modified CelI-based assays in one-well format for both marker development and genotyping. This serves as a very flexible and cost-effective approach.

Original languageEnglish
Pages (from-to)396-406
Number of pages11
JournalJournal of Heredity
Volume99
Issue number4
DOIs
Publication statusPublished - 2008

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Arabidopsis
introgression
Fertility
Quantitative Trait Loci
Population
Genome
Costs and Cost Analysis
Genes
genotyping
quantitative trait loci
flowering
genomics
loci
genome
assays
genes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Genetics
  • Genetics(clinical)

Cite this

Törjék, O., Meyer, R. C., Zehnsdorf, M., Teltow, M., Strompen, G., Witucka-Wall, H., ... Altmann, T. (2008). Construction and analysis of 2 reciprocal arabidopsis introgression line populations. Journal of Heredity, 99(4), 396-406. https://doi.org/10.1093/jhered/esn014

Construction and analysis of 2 reciprocal arabidopsis introgression line populations. / Törjék, O.; Meyer, Rhonda C.; Zehnsdorf, Maik; Teltow, Melanie; Strompen, Georg; Witucka-Wall, Hanna; Blacha, Anna; Altmann, Thomas.

In: Journal of Heredity, Vol. 99, No. 4, 2008, p. 396-406.

Research output: Contribution to journalArticle

Törjék, O, Meyer, RC, Zehnsdorf, M, Teltow, M, Strompen, G, Witucka-Wall, H, Blacha, A & Altmann, T 2008, 'Construction and analysis of 2 reciprocal arabidopsis introgression line populations', Journal of Heredity, vol. 99, no. 4, pp. 396-406. https://doi.org/10.1093/jhered/esn014
Törjék, O. ; Meyer, Rhonda C. ; Zehnsdorf, Maik ; Teltow, Melanie ; Strompen, Georg ; Witucka-Wall, Hanna ; Blacha, Anna ; Altmann, Thomas. / Construction and analysis of 2 reciprocal arabidopsis introgression line populations. In: Journal of Heredity. 2008 ; Vol. 99, No. 4. pp. 396-406.
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