CONSTITUTIVELY PHOTOMORPHOGENIC1 is required for the UV-B response in Arabidopsis

Attila Oravecz, Alexander Baumann, Z. Máté, Agnieszka Brzezinska, Jean Molinier, Edward J. Oakeley, E. Ádám, Eberhard Schäfer, Ferenc Nagy, Roman Ulm

Research output: Contribution to journalArticle

227 Citations (Scopus)

Abstract

CONSTITUTIVELY PHOTOMORPHOGENIC1 (COP1) is a negative regulator of photomorphogenesis in Arabidopsis thaliana. COP1 functions as an E3 ubiquitin ligase, targeting select proteins for proteasomal degradation in plants as well as in mammals. Among its substrates is the basic domain/leucine zipper (bZIP) transcription factor ELONGATED HYPOCOTYL5 (HY5), one of the key regulators of photomorphogenesis under all light qualities, including UV-B responses required for tolerance to this environmental threat. Here, we report that, in contrast with the situation in visible light, COP1 is a critical positive regulator of responses to low levels of UV-B. We show that in the cop1-4 mutant, flavonoid accumulation and genome-wide expression changes in response to UV-B are blocked to a large extent. COP1 is required for HY5 gene activation, and both COP1 and HY5 proteins accumulate in the nucleus under supplementary UV-B. SUPPRESSOR OF PHYTOCHROME A-105 family proteins (SPA1 to SPA4) that are required for COP1 function in dark and visible light are not essential in the response to UV-B. We conclude that COP1 performs a specific and novel role in the plants' photomorphogenic response to UV-B, coordinating HY5-dependent and -independent pathways, which eventually results in UV-B tolerance.

Original languageEnglish
Pages (from-to)1975-1990
Number of pages16
JournalPlant Cell
Volume18
Issue number8
DOIs
Publication statusPublished - Aug 2006

Fingerprint

Arabidopsis
ultraviolet radiation
Light
Phytochrome A
Genes
Basic-Leucine Zipper Transcription Factors
Leucine Zippers
Proteins
Mammals
Ubiquitin-Protein Ligases
photomorphogenesis
Flavonoids
Transcriptional Activation
Proteolysis
Chemical activation
Genome
Degradation
leucine zipper
Substrates
ubiquitin-protein ligase

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Oravecz, A., Baumann, A., Máté, Z., Brzezinska, A., Molinier, J., Oakeley, E. J., ... Ulm, R. (2006). CONSTITUTIVELY PHOTOMORPHOGENIC1 is required for the UV-B response in Arabidopsis. Plant Cell, 18(8), 1975-1990. https://doi.org/10.1105/tpc.105.040097

CONSTITUTIVELY PHOTOMORPHOGENIC1 is required for the UV-B response in Arabidopsis. / Oravecz, Attila; Baumann, Alexander; Máté, Z.; Brzezinska, Agnieszka; Molinier, Jean; Oakeley, Edward J.; Ádám, E.; Schäfer, Eberhard; Nagy, Ferenc; Ulm, Roman.

In: Plant Cell, Vol. 18, No. 8, 08.2006, p. 1975-1990.

Research output: Contribution to journalArticle

Oravecz, A, Baumann, A, Máté, Z, Brzezinska, A, Molinier, J, Oakeley, EJ, Ádám, E, Schäfer, E, Nagy, F & Ulm, R 2006, 'CONSTITUTIVELY PHOTOMORPHOGENIC1 is required for the UV-B response in Arabidopsis', Plant Cell, vol. 18, no. 8, pp. 1975-1990. https://doi.org/10.1105/tpc.105.040097
Oravecz A, Baumann A, Máté Z, Brzezinska A, Molinier J, Oakeley EJ et al. CONSTITUTIVELY PHOTOMORPHOGENIC1 is required for the UV-B response in Arabidopsis. Plant Cell. 2006 Aug;18(8):1975-1990. https://doi.org/10.1105/tpc.105.040097
Oravecz, Attila ; Baumann, Alexander ; Máté, Z. ; Brzezinska, Agnieszka ; Molinier, Jean ; Oakeley, Edward J. ; Ádám, E. ; Schäfer, Eberhard ; Nagy, Ferenc ; Ulm, Roman. / CONSTITUTIVELY PHOTOMORPHOGENIC1 is required for the UV-B response in Arabidopsis. In: Plant Cell. 2006 ; Vol. 18, No. 8. pp. 1975-1990.
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