Constitutive salicylic acid accumulation in pi4kIIIβ1β2 Arabidopsis plants stunts rosette but not root growth

Vladimír Šašek, Martin Janda, Elise Delage, Juliette Puyaubert, Anne Guivarc'h, Encarnación López Maseda, Petre I. Dobrev, José Caius, K. Bóka, Olga Valentová, Lenka Burketová, Alain Zachowski, Eric Ruelland

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Summary: Phospholipids have recently been found to be integral elements of hormone signalling pathways. An Arabidopsis thaliana double mutant in two type III phosphatidylinositol-4-kinases (PI4Ks), pi4kIIIβ1β2, displays a stunted rosette growth. The causal link between PI4K activity and growth is unknown. Using microarray analysis, quantitative reverse transcription polymerase chain reaction (RT-qPCR) and multiple phytohormone analysis by LC-MS we investigated the mechanism responsible for the pi4kIIIβ1β2 phenotype. The pi4kIIIβ1β2 mutant accumulated a high concentration of salicylic acid (SA), constitutively expressed SA marker genes including PR-1, and was more resistant to Pseudomonas syringae. pi4kIIIβ1β2 was crossed with SA signalling mutants eds1 and npr1 and SA biosynthesis mutant sid2 and NahG. The dwarf phenotype of pi4kIIIβ1β2 rosettes was suppressed in all four triple mutants. Whereas eds1 pi4kIIIβ1β2, sid2 pi4kIIIβ1β2 and NahG pi4kIIIβ1β2 had similar amounts of SA as the wild-type (WT), npr1pi4kIIIβ1β2 had more SA than pi4kIIIβ1β2 despite being less dwarfed. This indicates that PI4KIIIβ1 and PI4KIIIβ2 are genetically upstream of EDS1 and need functional SA biosynthesis and perception through NPR1 to express the dwarf phenotype. The slow root growth phenotype of pi4kIIIβ1β2 was not suppressed in any of the triple mutants. The pi4kIIIβ1β2 mutations together cause constitutive activation of SA signalling that is responsible for the dwarf rosette phenotype but not for the short root phenotype.

Original languageEnglish
Pages (from-to)805-816
Number of pages12
JournalNew Phytologist
Volume203
Issue number3
DOIs
Publication statusPublished - 2014

Fingerprint

Salicylic Acid
Arabidopsis
salicylic acid
root growth
Growth
Phenotype
phenotype
mutants
1-phosphatidylinositol 4-kinase
1-Phosphatidylinositol 4-Kinase
biosynthesis
Growth Disorders
Pseudomonas syringae
Plant Growth Regulators
Microarray Analysis
plant hormones
Reverse Transcription
Phospholipids
phospholipids
quantitative polymerase chain reaction

Keywords

  • Arabidopsis
  • Dwarf phenotype
  • Hormone transduction
  • Phosphatidylinositol-4-kinases (PI4Ks)
  • PR-1
  • Resistance
  • Salicylic acid (SA)

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Medicine(all)

Cite this

Šašek, V., Janda, M., Delage, E., Puyaubert, J., Guivarc'h, A., López Maseda, E., ... Ruelland, E. (2014). Constitutive salicylic acid accumulation in pi4kIIIβ1β2 Arabidopsis plants stunts rosette but not root growth. New Phytologist, 203(3), 805-816. https://doi.org/10.1111/nph.12822

Constitutive salicylic acid accumulation in pi4kIIIβ1β2 Arabidopsis plants stunts rosette but not root growth. / Šašek, Vladimír; Janda, Martin; Delage, Elise; Puyaubert, Juliette; Guivarc'h, Anne; López Maseda, Encarnación; Dobrev, Petre I.; Caius, José; Bóka, K.; Valentová, Olga; Burketová, Lenka; Zachowski, Alain; Ruelland, Eric.

In: New Phytologist, Vol. 203, No. 3, 2014, p. 805-816.

Research output: Contribution to journalArticle

Šašek, V, Janda, M, Delage, E, Puyaubert, J, Guivarc'h, A, López Maseda, E, Dobrev, PI, Caius, J, Bóka, K, Valentová, O, Burketová, L, Zachowski, A & Ruelland, E 2014, 'Constitutive salicylic acid accumulation in pi4kIIIβ1β2 Arabidopsis plants stunts rosette but not root growth', New Phytologist, vol. 203, no. 3, pp. 805-816. https://doi.org/10.1111/nph.12822
Šašek, Vladimír ; Janda, Martin ; Delage, Elise ; Puyaubert, Juliette ; Guivarc'h, Anne ; López Maseda, Encarnación ; Dobrev, Petre I. ; Caius, José ; Bóka, K. ; Valentová, Olga ; Burketová, Lenka ; Zachowski, Alain ; Ruelland, Eric. / Constitutive salicylic acid accumulation in pi4kIIIβ1β2 Arabidopsis plants stunts rosette but not root growth. In: New Phytologist. 2014 ; Vol. 203, No. 3. pp. 805-816.
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abstract = "Summary: Phospholipids have recently been found to be integral elements of hormone signalling pathways. An Arabidopsis thaliana double mutant in two type III phosphatidylinositol-4-kinases (PI4Ks), pi4kIIIβ1β2, displays a stunted rosette growth. The causal link between PI4K activity and growth is unknown. Using microarray analysis, quantitative reverse transcription polymerase chain reaction (RT-qPCR) and multiple phytohormone analysis by LC-MS we investigated the mechanism responsible for the pi4kIIIβ1β2 phenotype. The pi4kIIIβ1β2 mutant accumulated a high concentration of salicylic acid (SA), constitutively expressed SA marker genes including PR-1, and was more resistant to Pseudomonas syringae. pi4kIIIβ1β2 was crossed with SA signalling mutants eds1 and npr1 and SA biosynthesis mutant sid2 and NahG. The dwarf phenotype of pi4kIIIβ1β2 rosettes was suppressed in all four triple mutants. Whereas eds1 pi4kIIIβ1β2, sid2 pi4kIIIβ1β2 and NahG pi4kIIIβ1β2 had similar amounts of SA as the wild-type (WT), npr1pi4kIIIβ1β2 had more SA than pi4kIIIβ1β2 despite being less dwarfed. This indicates that PI4KIIIβ1 and PI4KIIIβ2 are genetically upstream of EDS1 and need functional SA biosynthesis and perception through NPR1 to express the dwarf phenotype. The slow root growth phenotype of pi4kIIIβ1β2 was not suppressed in any of the triple mutants. The pi4kIIIβ1β2 mutations together cause constitutive activation of SA signalling that is responsible for the dwarf rosette phenotype but not for the short root phenotype.",
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AU - Janda, Martin

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AU - Puyaubert, Juliette

AU - Guivarc'h, Anne

AU - López Maseda, Encarnación

AU - Dobrev, Petre I.

AU - Caius, José

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AB - Summary: Phospholipids have recently been found to be integral elements of hormone signalling pathways. An Arabidopsis thaliana double mutant in two type III phosphatidylinositol-4-kinases (PI4Ks), pi4kIIIβ1β2, displays a stunted rosette growth. The causal link between PI4K activity and growth is unknown. Using microarray analysis, quantitative reverse transcription polymerase chain reaction (RT-qPCR) and multiple phytohormone analysis by LC-MS we investigated the mechanism responsible for the pi4kIIIβ1β2 phenotype. The pi4kIIIβ1β2 mutant accumulated a high concentration of salicylic acid (SA), constitutively expressed SA marker genes including PR-1, and was more resistant to Pseudomonas syringae. pi4kIIIβ1β2 was crossed with SA signalling mutants eds1 and npr1 and SA biosynthesis mutant sid2 and NahG. The dwarf phenotype of pi4kIIIβ1β2 rosettes was suppressed in all four triple mutants. Whereas eds1 pi4kIIIβ1β2, sid2 pi4kIIIβ1β2 and NahG pi4kIIIβ1β2 had similar amounts of SA as the wild-type (WT), npr1pi4kIIIβ1β2 had more SA than pi4kIIIβ1β2 despite being less dwarfed. This indicates that PI4KIIIβ1 and PI4KIIIβ2 are genetically upstream of EDS1 and need functional SA biosynthesis and perception through NPR1 to express the dwarf phenotype. The slow root growth phenotype of pi4kIIIβ1β2 was not suppressed in any of the triple mutants. The pi4kIIIβ1β2 mutations together cause constitutive activation of SA signalling that is responsible for the dwarf rosette phenotype but not for the short root phenotype.

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KW - Resistance

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