Constitutive photomorphogenesis 1 and multiple phytoresectors control degradation of phytochrome interacting factor 3, a transcription factor required for light signaling in arabidopsis

Diana Bauer, András Viczián, Stefan Kircher, Tabea Nobis, Roland Nitschke, Tim Kunkel, Kishore C.S. Panigrahi, Éva Ádám, Erzsébet Fejes, Eberhard Schäfer, Ferenc Nagy

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299 Citations (Scopus)

Abstract

Light, in a quality- and quantity-dependent fashion, induces nuclear import of the plant photoreceptors phytochrome, promotes interaction of phytochrome A (phyA) and phyB with transcription factors including phytochrome interacting factor 3 (PIF3), and is thought to trigger a transcriptional cascade to regulate the expression of -2500 genes in Arabidopsis thaliana. Here, we show that controlled degradation of the transcription factor PIF3 is a major regulatory step in light signaling. We demonstrate that accumulation of PIF3 in the nucleus in dark requires constitutive photomorphogenesis 1 (COP1), a negative regulator of photomorphogenesis, and show that red (R) and far-red light (FR) induce rapid degradation of the PIF3 protein. This process is controlled by the concerted action of the R/FR absorbing phyA, phyB, and phyD photoreceptors, and it is not affected by COPI. Rapid light-induced degradation of PIF3 indicates that interaction of PIF3 with these phytochrome species is transient. In addition, we provide evidence that the pocf mutant, a postulated PIF3 overexpressor that displays hypersensitivity to R but not to FR, lacks detectable amounts of the PIF3 protein. Thus, we propose that PIF3 acts transiently, and its major function is to mediate phytochrome-induced signaling during the developmental switch from skotomorphogenesis to photomorphogenesis and/or dark to light transitions.

Original languageEnglish
Pages (from-to)1433-1445
Number of pages13
JournalPlant Cell
Volume16
Issue number6
DOIs
Publication statusPublished - Jun 2004

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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    Bauer, D., Viczián, A., Kircher, S., Nobis, T., Nitschke, R., Kunkel, T., Panigrahi, K. C. S., Ádám, É., Fejes, E., Schäfer, E., & Nagy, F. (2004). Constitutive photomorphogenesis 1 and multiple phytoresectors control degradation of phytochrome interacting factor 3, a transcription factor required for light signaling in arabidopsis. Plant Cell, 16(6), 1433-1445. https://doi.org/10.1105/tpc.021568