Constitutive intracellular expression and activation-induced cell surface up-regulation of CD44v3 in human T lymphocytes

Csaba Forster-Horváth, József Bocsi, E. Rásó, Tamás I. Orbán, E. Oláh, J. Tímár, A. Ladányi

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The cell adhesion molecule CD44 exists in multiple isoforms generated by alternative RNA splicing. Increased expression of CD44 isoforms containing exon v6 and v9 has been reported to be associated with the activated state of T lymphocytes. Using monoclonal antibodies against variant exon products we studied the expression of another variant exon, v3 on resting and in vitro activated human peripheral blood T cells. We found that CD44v3, in parallel with CD44v6, is up-regulated at the surface of normal T cells stimulated by anti-CD3 antibody or by the phorbol ester PMA, as well as on PMA-stimulated T cell leukemia lines CCRF-CEM and MOLT-4. Beside the cell surface, we demonstrated CD44v3 intracellularly in both resting and activated T cells by flow cytometry and immunomorphology. Reverse transcription-PCR and Western blot analyses confirmed the constitutive expression of CD44v3 in these cells. The increase in the cell surface expression of CD44v3 on stimulated T lymphocytes was inhibited by cycloheximide and brefeldin A, indicating the requirement of de novo protein synthesis and endoplasmic reticulum Golgi transport. Our studies establish CD44v3 as an additional activation marker for human T cells, with a yet unidentified function.

Original languageEnglish
Pages (from-to)600-608
Number of pages9
JournalEuropean Journal of Immunology
Volume31
Issue number2
DOIs
Publication statusPublished - 2001

Fingerprint

Up-Regulation
T-Lymphocytes
Exons
Protein Isoforms
T-Cell Leukemia
Brefeldin A
Alternative Splicing
Cell Adhesion Molecules
Phorbol Esters
Cycloheximide
Endoplasmic Reticulum
Reverse Transcription
Anti-Idiotypic Antibodies
Blood Cells
Flow Cytometry
Western Blotting
Monoclonal Antibodies
Cell Line
Polymerase Chain Reaction
Proteins

Keywords

  • Activation
  • CD44v3
  • Human
  • T lymphocyte

ASJC Scopus subject areas

  • Immunology

Cite this

Constitutive intracellular expression and activation-induced cell surface up-regulation of CD44v3 in human T lymphocytes. / Forster-Horváth, Csaba; Bocsi, József; Rásó, E.; Orbán, Tamás I.; Oláh, E.; Tímár, J.; Ladányi, A.

In: European Journal of Immunology, Vol. 31, No. 2, 2001, p. 600-608.

Research output: Contribution to journalArticle

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