Conserved CDC20 cell cycle functions are carried out by two of the five isoforms in arabidopsis thaliana

Zoltán Kevei, Mikhail Baloban, Olivier da Ines, Hilda Tiricz, Alexandra Kroll, Krzysztof Regulski, Peter Mergaert, É. Kondorosi

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Background: The CDC20 and Cdh1/CCS52 proteins are substrate determinants and activators of the Anaphase Promoting Complex/Cyclosome (APC/C) E3 ubiquitin ligase and as such they control the mitotic cell cycle by targeting the degradation of various cell cycle regulators. In yeasts and animals the main CDC20 function is the destruction of securin and mitotic cyclins. Plants have multiple CDC20 gene copies whose functions have not been explored yet. In Arabidopsis thaliana there are five CDC20 isoforms and here we aimed at defining their contribution to cell cycle regulation, substrate selectivity and plant development. Methodology/Principal Findings: Studying the gene structure and phylogeny of plant CDC20s, the expression of the five AtCDC20 gene copies and their interactions with the APC/C subunit APC10, the CCS52 proteins, components of the mitotic checkpoint complex (MCC) and mitotic cyclin substrates, conserved CDC20 functions could be assigned for AtCDC20.1 and AtCDC20.2. The other three intron-less genes were silent and specific for Arabidopsis. We show that AtCDC20.1 and AtCDC20.2 are components of the MCC and interact with mitotic cyclins with unexpected specificity. AtCDC20.1 and AtCDC20.2 are expressed in meristems, organ primordia and AtCDC20.1 also in pollen grains and developing seeds. Knocking down both genes simultaneously by RNAi resulted in severe delay in plant development and male sterility. In these lines, the meristem size was reduced while the cell size and ploidy levels were unaffected indicating that the lower cell number and likely slowdown of the cell cycle are the cause of reduced plant growth. Conclusions/Significance: The intron-containing CDC20 gene copies provide conserved and redundant functions for cell cycle progression in plants and are required for meristem maintenance, plant growth and male gametophyte formation. The Arabidopsis-specific intron-less genes are possibly "retrogenes" and have hitherto undefined functions or are pseudogenes.

Original languageEnglish
Article numbere20618
JournalPLoS One
Volume6
Issue number6
DOIs
Publication statusPublished - 2011

Fingerprint

Arabidopsis
cell cycle
Cell Cycle
Protein Isoforms
Arabidopsis thaliana
Genes
Cells
Cyclins
Meristem
cyclins
Anaphase-Promoting Complex-Cyclosome
meristems
Introns
genes
M Phase Cell Cycle Checkpoints
introns
Plant Development
Cdh1 Proteins
plant development
Securin

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Conserved CDC20 cell cycle functions are carried out by two of the five isoforms in arabidopsis thaliana. / Kevei, Zoltán; Baloban, Mikhail; da Ines, Olivier; Tiricz, Hilda; Kroll, Alexandra; Regulski, Krzysztof; Mergaert, Peter; Kondorosi, É.

In: PLoS One, Vol. 6, No. 6, e20618, 2011.

Research output: Contribution to journalArticle

Kevei, Z, Baloban, M, da Ines, O, Tiricz, H, Kroll, A, Regulski, K, Mergaert, P & Kondorosi, É 2011, 'Conserved CDC20 cell cycle functions are carried out by two of the five isoforms in arabidopsis thaliana', PLoS One, vol. 6, no. 6, e20618. https://doi.org/10.1371/journal.pone.0020618
Kevei, Zoltán ; Baloban, Mikhail ; da Ines, Olivier ; Tiricz, Hilda ; Kroll, Alexandra ; Regulski, Krzysztof ; Mergaert, Peter ; Kondorosi, É. / Conserved CDC20 cell cycle functions are carried out by two of the five isoforms in arabidopsis thaliana. In: PLoS One. 2011 ; Vol. 6, No. 6.
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AU - Baloban, Mikhail

AU - da Ines, Olivier

AU - Tiricz, Hilda

AU - Kroll, Alexandra

AU - Regulski, Krzysztof

AU - Mergaert, Peter

AU - Kondorosi, É.

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