Conservation of the Caenorhabditis elegans timing gene clk-1 from yeast to human

A gene required for ubiquinone biosynthesis with potential implications for aging

Z. Vajó, Lynn M. King, Tanya Jonassen, Douglas J. Wilkin, Nicola Ho, Arnold Munnich, Catherine F. Clarke, Clair A. Francomano

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Mutations in the Caenorhabditis elegans gene clk-1 have a major effect on slowing development and increasing life span. The Saccharomyces cerevisiae homolog COQ7 encodes a mitochondrial protein involved in ubiquinone biosynthesis and, hence, is required for respiration and gluconeogenesis. In this study, RT-PCR and 5' RACE were used to isolate both human and mouse clk- 1/COQ7 homologs. Human CLK-1 was mapped to Chr 16(p12-13.1) by Radiation Hybrid (RH) and fluorescence in situ hybridization (FISH) methods. The number and location of human CLK1 introns were determined, and the location of introns II and IV are the same as in C. elegans. Northern blot analysis showed that three different isoforms of CLK-1 mRNA are present in several tissues and that the isoforms differ in the amount of expression. The functional equivalence of human CLK-1 to the yeast COQ7 homolog was tested by introducing either a single or multicopy plasmid containing human CLK-1 cDNA into yeast coq7 deletion strains and assaying for growth on a nonfermentable carbon source. The human CLK-1 gene was able to functionally complement yeast coq7 deletion mutants. The protein similarities and the conservation of function of the CLK-1/clk-I/COQ7 gene products suggest a potential link between the production of ubiquinone and aging.

Original languageEnglish
Pages (from-to)1000-1004
Number of pages5
JournalMammalian Genome
Volume10
Issue number10
DOIs
Publication statusPublished - 1999

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Ubiquinone
Caenorhabditis elegans
Yeasts
Genes
Introns
Protein Isoforms
Radiation Hybrid Mapping
Gluconeogenesis
Mitochondrial Proteins
Fluorescence In Situ Hybridization
Northern Blotting
Saccharomyces cerevisiae
Respiration
Plasmids
Carbon
Complementary DNA
Polymerase Chain Reaction
Messenger RNA
Mutation
Growth

ASJC Scopus subject areas

  • Genetics

Cite this

Conservation of the Caenorhabditis elegans timing gene clk-1 from yeast to human : A gene required for ubiquinone biosynthesis with potential implications for aging. / Vajó, Z.; King, Lynn M.; Jonassen, Tanya; Wilkin, Douglas J.; Ho, Nicola; Munnich, Arnold; Clarke, Catherine F.; Francomano, Clair A.

In: Mammalian Genome, Vol. 10, No. 10, 1999, p. 1000-1004.

Research output: Contribution to journalArticle

Vajó, Z. ; King, Lynn M. ; Jonassen, Tanya ; Wilkin, Douglas J. ; Ho, Nicola ; Munnich, Arnold ; Clarke, Catherine F. ; Francomano, Clair A. / Conservation of the Caenorhabditis elegans timing gene clk-1 from yeast to human : A gene required for ubiquinone biosynthesis with potential implications for aging. In: Mammalian Genome. 1999 ; Vol. 10, No. 10. pp. 1000-1004.
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