A Kupffer-sejt gátlásának hatása az endotoxin indukálta gyulladásos válaszreakciókra és a máj mikrokeringési változásaira kísérletes obstructiós icterusban.

Translated title of the contribution: Consequences of Kupffer cell blockade on endotoxin-induced inflammatory and hepatic microcirculatory reactions during experimental biliary obstruction

Szabolcs Abrahám, Andrea Szabó, Attila Paszt, Erno Duda, György Lázár, György Lázár

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

INTRODUCTION/AIMS: In the clinical practice, biliary obstruction often leads to septic complications causing systemic and hepatic inflammatory reactions, which increases mortality and morbidity. Hepatic Kupffer cells (KC) play a pivotal role in this process. Herein we examined the consequences of bile duct ligation during endotoxaemia and the effects of KCs. MATERIAL AND METHODS: In the first part of our experiment, the survival rate of male Wistar rats in 48-hr endotoxaemia with or without bile duct ligation was assessed. Time-dependent changes in pro-inflammatory TNF-alpha and IL-6 levels were also monitored. In the second series, hepatic capillary perfusion, neutrophil-endothelial interactions and KC activity were assessed using fluorescence intravital videomicroscopy. KC blockade was induced by gadolinium chloride (GdCl3) pretreatment. RESULTS: Serum TNF-alpha and IL-6 levels were significantly increased in early endotoxaemia. Survival rate was deteriorated, while TNF-alpha and IL-6 releases, KC activity and leukocyte activation were increased if obstructive jaundice was also induced. KC blockade improved survival and reduced TNF-alpha and IL-6 productions without ameliorating perfusion failure. CONCLUSIONS: In the presence of biliary obstruction, inflammatory and microcirculatory consequences of endotoxaemia are enhanced. The alleviating effect of KC blockade may underline the pathophysiological role of KCs in these conditions.

Translated title of the contributionConsequences of Kupffer cell blockade on endotoxin-induced inflammatory and hepatic microcirculatory reactions during experimental biliary obstruction
Original languageHungarian
Pages (from-to)298-303
Number of pages6
JournalMagyar sebészet
Volume62
Issue number5
DOIs
Publication statusPublished - Oct 2009

ASJC Scopus subject areas

  • Medicine(all)

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