Connection between redox homeostasis and metal ion homeostasis in hepatic ischemia reperfusion injury of the rat

Research output: Contribution to journalArticle

Abstract

Oxidative damage connected with ischemia reperfusion occurs during liver resection and transplantation. The aim was to elucidate the correlation between alterations of the redox defensive system and metal element concentrations in ischemia reperfusion injury. Wistar rats were divided into 4 groups: control, sham-operated, ischemic and reperfusion groups. Hepatic ischemia was induced for 45 min followed by 15 min of reperfusion. Chemiluminescent intensity, H-donating ability, reducing power, SH group concentration, total antioxidant status, glutathione peroxidase and superoxide dismutase activity were detected by luminometry and spectrophotometry. Blood parameters (Hitachi 717 Analyzer) as well as Ca, Cu, Fe, Mg, Mn, P, S and Zn concentrations of liver with ICP-OES were also determined. Se content was determined by a cathodic stripping voltametric method. H-donating ability and reducing power decreased and glutathione peroxidase level of the liver were significantly lower during reperfusion than in the sham-operated group. The same tendency could be observed in the level of superoxide dismutase. The changes in the metal element concentration are essential for cellular pathways, for example, significant decrease in Cu, Zn, Mn and Se content may serve as an explanation for decreased glutathione peroxidase and superoxide dismutase activities. Changes in Ca, Mg, P and S concentrations during ischemia reperfusion processes also show the important role of these elements in the tissue regeneration. Significant disturbance of the antioxidant balance could be detected, which was related to the alterations of metal element concentration in the liver tissue.

Original languageEnglish
Pages (from-to)292-298
Number of pages7
JournalTrace Elements and Electrolytes
Volume23
Issue number4
Publication statusPublished - Dec 2006

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Reperfusion Injury
Liver
Reperfusion
Oxidation-Reduction
Metal ions
Rats
Homeostasis
Glutathione Peroxidase
Metals
Ions
Superoxide Dismutase
Ischemia
Antioxidants
Tissue regeneration
Spectrophotometry
Blood
Liver Transplantation
Tissue
Wistar Rats
Regeneration

Keywords

  • Free radicals
  • Ischemia reperfusion
  • Liver
  • Metal elements

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

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title = "Connection between redox homeostasis and metal ion homeostasis in hepatic ischemia reperfusion injury of the rat",
abstract = "Oxidative damage connected with ischemia reperfusion occurs during liver resection and transplantation. The aim was to elucidate the correlation between alterations of the redox defensive system and metal element concentrations in ischemia reperfusion injury. Wistar rats were divided into 4 groups: control, sham-operated, ischemic and reperfusion groups. Hepatic ischemia was induced for 45 min followed by 15 min of reperfusion. Chemiluminescent intensity, H-donating ability, reducing power, SH group concentration, total antioxidant status, glutathione peroxidase and superoxide dismutase activity were detected by luminometry and spectrophotometry. Blood parameters (Hitachi 717 Analyzer) as well as Ca, Cu, Fe, Mg, Mn, P, S and Zn concentrations of liver with ICP-OES were also determined. Se content was determined by a cathodic stripping voltametric method. H-donating ability and reducing power decreased and glutathione peroxidase level of the liver were significantly lower during reperfusion than in the sham-operated group. The same tendency could be observed in the level of superoxide dismutase. The changes in the metal element concentration are essential for cellular pathways, for example, significant decrease in Cu, Zn, Mn and Se content may serve as an explanation for decreased glutathione peroxidase and superoxide dismutase activities. Changes in Ca, Mg, P and S concentrations during ischemia reperfusion processes also show the important role of these elements in the tissue regeneration. Significant disturbance of the antioxidant balance could be detected, which was related to the alterations of metal element concentration in the liver tissue.",
keywords = "Free radicals, Ischemia reperfusion, Liver, Metal elements",
author = "L. V{\'a}li and K. Szentmih{\'a}lyi and G. Taba and H. F{\'e}bel and I. Kocsis and A. Lugasi and A. Bl{\'a}zovics",
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T1 - Connection between redox homeostasis and metal ion homeostasis in hepatic ischemia reperfusion injury of the rat

AU - Váli, L.

AU - Szentmihályi, K.

AU - Taba, G.

AU - Fébel, H.

AU - Kocsis, I.

AU - Lugasi, A.

AU - Blázovics, A.

PY - 2006/12

Y1 - 2006/12

N2 - Oxidative damage connected with ischemia reperfusion occurs during liver resection and transplantation. The aim was to elucidate the correlation between alterations of the redox defensive system and metal element concentrations in ischemia reperfusion injury. Wistar rats were divided into 4 groups: control, sham-operated, ischemic and reperfusion groups. Hepatic ischemia was induced for 45 min followed by 15 min of reperfusion. Chemiluminescent intensity, H-donating ability, reducing power, SH group concentration, total antioxidant status, glutathione peroxidase and superoxide dismutase activity were detected by luminometry and spectrophotometry. Blood parameters (Hitachi 717 Analyzer) as well as Ca, Cu, Fe, Mg, Mn, P, S and Zn concentrations of liver with ICP-OES were also determined. Se content was determined by a cathodic stripping voltametric method. H-donating ability and reducing power decreased and glutathione peroxidase level of the liver were significantly lower during reperfusion than in the sham-operated group. The same tendency could be observed in the level of superoxide dismutase. The changes in the metal element concentration are essential for cellular pathways, for example, significant decrease in Cu, Zn, Mn and Se content may serve as an explanation for decreased glutathione peroxidase and superoxide dismutase activities. Changes in Ca, Mg, P and S concentrations during ischemia reperfusion processes also show the important role of these elements in the tissue regeneration. Significant disturbance of the antioxidant balance could be detected, which was related to the alterations of metal element concentration in the liver tissue.

AB - Oxidative damage connected with ischemia reperfusion occurs during liver resection and transplantation. The aim was to elucidate the correlation between alterations of the redox defensive system and metal element concentrations in ischemia reperfusion injury. Wistar rats were divided into 4 groups: control, sham-operated, ischemic and reperfusion groups. Hepatic ischemia was induced for 45 min followed by 15 min of reperfusion. Chemiluminescent intensity, H-donating ability, reducing power, SH group concentration, total antioxidant status, glutathione peroxidase and superoxide dismutase activity were detected by luminometry and spectrophotometry. Blood parameters (Hitachi 717 Analyzer) as well as Ca, Cu, Fe, Mg, Mn, P, S and Zn concentrations of liver with ICP-OES were also determined. Se content was determined by a cathodic stripping voltametric method. H-donating ability and reducing power decreased and glutathione peroxidase level of the liver were significantly lower during reperfusion than in the sham-operated group. The same tendency could be observed in the level of superoxide dismutase. The changes in the metal element concentration are essential for cellular pathways, for example, significant decrease in Cu, Zn, Mn and Se content may serve as an explanation for decreased glutathione peroxidase and superoxide dismutase activities. Changes in Ca, Mg, P and S concentrations during ischemia reperfusion processes also show the important role of these elements in the tissue regeneration. Significant disturbance of the antioxidant balance could be detected, which was related to the alterations of metal element concentration in the liver tissue.

KW - Free radicals

KW - Ischemia reperfusion

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