Congenital absence of vasopressin and age-dependent changes in ACTH and corticosterone stress responses in rats

D. Zelena, I. Barna, O. Pintér, B. Klausz, J. Varga, G. Makara

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The hypothalamic components of the hypothalamo-pituitary-adrenal axis (HPA) are corticotropin-releasing hormone (CRH) and vasopressin. To test the hypothesis that HPA regulation changes with age, we compared ether and bacterial lipopolysaccharide (LPS) injection induced stress reactions in adult and 10-day-old Brattleboro rats, which naturally lack vasopressin owing to mutation of the gene (di/di). The LPS stimulus was used also with V1b receptor antagonist pretreatment (SSR149415). In adult di/di or V1b pretreated rats, we observed normal pituitary and adrenocortical secretory responses, while in all 10-day-old rats stress-induced serum corticosterone increases were marked, but adrenocorticotropin (ACTH) increases were significantly smaller. Compared to control pups the adenohypophysis of the 10-day-old di/di rats responded normally to CRH, but their adrenal glands were hyper-responsive to ACTH, while in adults there was greater secretion at both levels with no difference between the genotypes. The serum transcortin level was higher in adults than pups, with the di/di pups having higher transcortin levels than controls. Hence, using the same stressors in adults and pups with both a genetic model and pharmacological pretreatment, we have shown that the role of vasopressin in ACTH regulation is more important during the neonatal period than in adulthood. Blunted hypophysial sensitivity to CRH and similar adrenal gland sensitivity to ACTH in the pups compared to adults suggest that hypothalamic factors could be responsible for the neonatal stress hyporesponsive period.

Original languageEnglish
Pages (from-to)420-430
Number of pages11
JournalStress
Volume14
Issue number4
DOIs
Publication statusPublished - Jul 2011

Fingerprint

Corticosterone
Vasopressins
Adrenocorticotropic Hormone
Corticotropin-Releasing Hormone
Transcortin
Adrenal Glands
Lipopolysaccharides
Brattleboro Rats
Anterior Pituitary Gland
Genetic Models
Serum
Ether
Genotype
Pharmacology
Mutation
Injections
Genes

Keywords

  • CBG
  • corticosterone
  • ether exposure
  • LPS
  • stress hyporesponsive period
  • V1b antagonist

ASJC Scopus subject areas

  • Behavioral Neuroscience
  • Endocrine and Autonomic Systems
  • Physiology
  • Psychiatry and Mental health
  • Neuropsychology and Physiological Psychology

Cite this

Congenital absence of vasopressin and age-dependent changes in ACTH and corticosterone stress responses in rats. / Zelena, D.; Barna, I.; Pintér, O.; Klausz, B.; Varga, J.; Makara, G.

In: Stress, Vol. 14, No. 4, 07.2011, p. 420-430.

Research output: Contribution to journalArticle

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