Conformational adaptability of the terminal regions of flagellin

F. Vonderviszt, M. Sonoyama, M. Tasumi, K. Namba

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Secondary structure formation in the disordered terminal regions of flagellin were studied by circular dichroic (CD) spectroscopy, Fourier transform infrared spectroscopy, and x-ray diffraction. The terminal regions of flagellin are known to form α-helical bundles upon polymerization into flagellar filaments. We found from comparative CD studies of flagellin and its F40 tryptic fragment that a highly α-helical conformation can be induced and stabilized in the terminal regions in 2,2,2-trifluoroethanol (TFE) containing solutions, which is known to promote intra-molecular hydrogen bonding. Two oligopeptides, N(37-61) and C(470-494), each corresponding to a portion of terminal regions and predicted to have a high α-helix forming potential, were synthesized and studied. Both peptides were disordered in an aqueous environment, but they showed a strong tendency to assume α-helical structure in solutions containing TFE. On the other hand, peptides were found to form transparent gels at high concentrations (> 15 mg/ml) and all three methods confirmed that the peptides become ordered into a predominantly β structure upon gel formation. Our results show that large segments of the disordered terminal regions of flagellin can adopt α-helical as well as β structure depending on the environmental conditions. This high degree of conformational adaptability may be reflecting some unique characteristics of the flagellin termini, which are involved in self-assembly and polymorphism of flagellar filament.

Original languageEnglish
Pages (from-to)1672-1677
Number of pages6
JournalBiophysical Journal
Volume63
Issue number6
Publication statusPublished - 1992

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Flagellin
Trifluoroethanol
Peptides
Gels
Oligopeptides
Fourier Transform Infrared Spectroscopy
Hydrogen Bonding
Polymerization
Spectrum Analysis
X-Rays

ASJC Scopus subject areas

  • Biophysics

Cite this

Vonderviszt, F., Sonoyama, M., Tasumi, M., & Namba, K. (1992). Conformational adaptability of the terminal regions of flagellin. Biophysical Journal, 63(6), 1672-1677.

Conformational adaptability of the terminal regions of flagellin. / Vonderviszt, F.; Sonoyama, M.; Tasumi, M.; Namba, K.

In: Biophysical Journal, Vol. 63, No. 6, 1992, p. 1672-1677.

Research output: Contribution to journalArticle

Vonderviszt, F, Sonoyama, M, Tasumi, M & Namba, K 1992, 'Conformational adaptability of the terminal regions of flagellin', Biophysical Journal, vol. 63, no. 6, pp. 1672-1677.
Vonderviszt, F. ; Sonoyama, M. ; Tasumi, M. ; Namba, K. / Conformational adaptability of the terminal regions of flagellin. In: Biophysical Journal. 1992 ; Vol. 63, No. 6. pp. 1672-1677.
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