Confirmation of a novel siadenovirus species detected in raptors

Partial sequence and phylogenetic analysis

Endre R. Kovács, M. Benkó

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Partial genome characterisation of a novel adenovirus, found recently in organ samples of multiple species of dead birds of prey, was carried out by sequence analysis of PCR-amplified DNA fragments. The virus, named as raptor adenovirus 1 (RAdV-1), has originally been detected by a nested PCR method with consensus primers targeting the adenoviral DNA polymerase gene. Phylogenetic analysis with the deduced amino acid sequence of the small PCR product has implied a new siadenovirus type present in the samples. Since virus isolation attempts remained unsuccessful, further characterisation of this putative novel siadenovirus was carried out with the use of PCR on the infected organ samples. The DNA sequence of the central genome part of RAdV-1, encompassing nine full (pTP, 52K, pIIIa, III, pVII, pX, pVI, hexon, protease) and two partial (DNA polymerase and DBP) genes and exceeding 12 kb pairs in size, was determined. Phylogenetic tree reconstructions, based on several genes, unambiguously confirmed the preliminary classification of RAdV-1 as a new species within the genus Siadenovirus. Further study of RAdV-1 is of interest since it represents a rare adenovirus genus of yet undetermined host origin.

Original languageEnglish
Pages (from-to)64-70
Number of pages7
JournalVirus Research
Volume140
Issue number1-2
DOIs
Publication statusPublished - Mar 2009

Fingerprint

Siadenovirus
Raptors
Adenoviridae
Sequence Analysis
Polymerase Chain Reaction
DNA-Directed DNA Polymerase
Genome
Genes
Viruses
Amino Acid Sequence
Peptide Hydrolases

Keywords

  • Genome sequencing
  • PCR
  • Phylogeny
  • Raptor adenovirus 1
  • Siadenovirus

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Cancer Research

Cite this

Confirmation of a novel siadenovirus species detected in raptors : Partial sequence and phylogenetic analysis. / Kovács, Endre R.; Benkó, M.

In: Virus Research, Vol. 140, No. 1-2, 03.2009, p. 64-70.

Research output: Contribution to journalArticle

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