Concanavalin A-agarose removes mannan impurities from an extracellularly expressed Pichia pastoris recombinant protein

Małgorzata Palczewska, Gyula Batta, Patrick Groves

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Pichia pastoris secretes few native proteins. However, the more than 1 g 1-1 of extracellularly expressed mannan interfered with the purification of our extracellularly expressed, non-glycosylated recombinant protein. Concanavalin A-agarose removed more than 95% of the unwanted mannan as monitored by phenol reaction. A 13C-based NMR assay confirmed this improvement. Concanavalin A-agarose can assist the purification of extracellular expressed, non-glycosylated proteins from yeasts.

Original languageEnglish
Pages (from-to)783-792
Number of pages10
JournalCellular and Molecular Biology Letters
Volume8
Issue number3
Publication statusPublished - Jan 1 2003

Keywords

  • Concanavalin A-Agarose
  • Extracellular Expressed Protein
  • Mannan
  • Pichia pastoris

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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