Compromised bone healing following spacer removal in a rat femoral defect model

G. Skaliczki, M. Weszl, K. Schandl, T. Major, M. Kovács, J. Skaliczki, H. Redl, M. Szendroi, K. Szigeti, D. Máté, C. S. Dobó-Nagy, Z. S. Lacza

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6 Citations (Scopus)


Purpose: The clinical demand for bone grafting materials necessitated the development of animal models. Critical size defect model has been criticized recently, mainly for its inaccuracy. Our objective was to develop a dependable animal model that would provide compromised bone healing, and would allow the investigation of bone substitutes. Methods: In the first group a critical size defect was created in the femur of adult male Wistar rats, and a non-critical defect in the remaining animals (Groups II, III and IV). The defect was left empty in group II, while in groups III and IV a spacer was interposed into the gap. Osteoblast activity was evaluated by NanoSPECT/CT imaging system. New bone formation and assessment of a union or non-union was observed by μCT and histology. Results: The interposition model proved to be highly reproducible and provided a bone defect with compromised bone healing. Significant bone regeneration processes were observed four weeks after removal of the spacer. Conclusion: Our results have shown that when early bone healing is inhibited by the physical interposition of a spacer, the regeneration process is compromised for a further 4 weeks and results in a bone defect during the time-course of the study.

Original languageEnglish
Pages (from-to)223-232
Number of pages10
JournalActa physiologica Hungarica
Issue number2
Publication statusPublished - Jun 1 2012


  • Critical size bone defect
  • Delayed healing
  • Fracture
  • Non-union

ASJC Scopus subject areas

  • Physiology (medical)

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    Skaliczki, G., Weszl, M., Schandl, K., Major, T., Kovács, M., Skaliczki, J., Redl, H., Szendroi, M., Szigeti, K., Máté, D., Dobó-Nagy, C. S., & Lacza, Z. S. (2012). Compromised bone healing following spacer removal in a rat femoral defect model. Acta physiologica Hungarica, 99(2), 223-232.