Comprehensive regression analysis of hepatitis B virus X antigen level and anti-HBx antibody titer in the sera of patients with HBV infection

J. Pál, Zoltán Nyárády, Ilona Marczinovits, A. Pár, Younes Saleh Ali, G. Berencsi, K. Kvell, P. Németh

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Although the pathogenetic significance of hepatitis B virus x protein (HBxAg) in chronic hepatitis, liver cirrhosis, and primary hepatocellular carcinoma has already been studied, the comparative analyses of both the actual serum HBxAg levels and antibody production against various HBx epitopes have been examined to lesser extent. We have simultaneously investigated the relationship between antibody production (IgG and IgM) against the HBxAg fragments and HBxAg level in the sera of patients with acute (14) or chronic hepatitis (80) and symptomless carriers (12). A recently developed sandwich-type ELISA was used for the quantitative measurements of HBxAg. Overlapping recombinant and synthetic antigens were used to map the fine epitope specificities of circulating anti-HBx antibodies. In acute hepatitis, we have found high and homogenous correlation in the IgM type immune responses against all the examined HBxAg regions. Moreover, strong correlation has been observed between IgG type immune responses to a characteristic C-terminal region (C1: 79-117) and the longest fragment (X: 10-143). Moderate correlation has been found between HBxAg concentration and the IgG type anti-HBx antibody levels against C-terminus of HBxAg in patients with chronic hepatitis. In the case of symptomless carriers, there were also demonstrable associations in the immune responses against the C-terminal sequences; however, significant correlations were found for antibody production against the N-terminal region as well. The examinations show that the C-terminal sequence, responsible for transactivation, promotes an efficient IgG antibody response in all three groups of patients, whereas the negative regulator N-terminal part of the HBxAg molecule for the most part does not trigger antibody production. This suggests that the immune responses against various - biologically active - epitopes of the HBxAg may have a different role in the pathogenesis of hepatitis and may be used as prognostic markers in human HBV infections.

Original languageEnglish
Pages (from-to)34-40
Number of pages7
JournalPathology and Oncology Research
Volume12
Issue number1
DOIs
Publication statusPublished - 2006

Fingerprint

Antibody Formation
Anti-Idiotypic Antibodies
Regression Analysis
Chronic Hepatitis
Immunoglobulin G
Epitopes
Infection
Serum
Hepatitis
Immunoglobulin M
Synthetic Vaccines
Hepatitis B virus
Liver Cirrhosis
Transcriptional Activation
Hepatocellular Carcinoma
Enzyme-Linked Immunosorbent Assay
hepatitis B virus X protein
Proteins

Keywords

  • Antibody response
  • Epitope mapping
  • HBxAg
  • Hepatitis B virus
  • Immunoserology

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Pathology and Forensic Medicine

Cite this

Comprehensive regression analysis of hepatitis B virus X antigen level and anti-HBx antibody titer in the sera of patients with HBV infection. / Pál, J.; Nyárády, Zoltán; Marczinovits, Ilona; Pár, A.; Ali, Younes Saleh; Berencsi, G.; Kvell, K.; Németh, P.

In: Pathology and Oncology Research, Vol. 12, No. 1, 2006, p. 34-40.

Research output: Contribution to journalArticle

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