Comprehensive phospholipid and sphingomyelin profiling of different brain regions in mouse model of anxiety disorder using online two-dimensional (HILIC/RP)-LC/MS method

R. Berkecz, Ferenc Tömösi, Tímea Körmöczi, Viktor Szegedi, János Horváth, T. Janáky

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A novel online system including two-dimensional liquid chromatography coupled to high-resolution mass spectrometry (2D-LC/MS) was developed and applied for comprehensive phospholipid (PL) and sphingomyelin (SM) profiling of dorsal hippocampus (DHPC), ventral (VHPC) and prefrontal cortex (PFC) brain regions in a mouse model of anxiety disorder. In the first dimension, lipid classes were distinguished by hydrophilic interaction liquid chromatography (HILIC), while the second dimensional separation of individual PL and SM species was achieved by reversed-phase (RP) chromatography. For the enrichment of lipid species in diluted HILIC effluent, two RP trapping columns were used separately. The developed fully-automated 2D method allowed the quantitative analysis of over 150 endogenous PL and SM species in mouse brain regions within 40 min. The developed method was applied in a pilot study, which aimed to find alteration of PL and SM composition in a mouse model of anxiety disorder. In the case of 37 PL and SM species, significant differences were observed between high anxiety-related behavior (AX) and low anxiety-related behavior (nAX) mice. In mice having elevated anxiety, the most typical trend was the downregulation of PL species, in particular, in VHPC.

Original languageEnglish
Pages (from-to)308-317
Number of pages10
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume149
DOIs
Publication statusPublished - Feb 5 2018

Fingerprint

Sphingomyelins
Liquid chromatography
Reverse-Phase Chromatography
Anxiety Disorders
Hydrophobic and Hydrophilic Interactions
Brain
Phospholipids
Liquid Chromatography
Anxiety
Online Systems
Lipids
Online systems
Chromatography
Prefrontal Cortex
Chemical analysis
Mass spectrometry
Effluents
Hippocampus
Mass Spectrometry
Down-Regulation

Keywords

  • 2D-LC/MS
  • Anxiety disorder
  • Brain
  • Lipidomics
  • Phospholipid
  • Sphingomyelin

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

Cite this

Comprehensive phospholipid and sphingomyelin profiling of different brain regions in mouse model of anxiety disorder using online two-dimensional (HILIC/RP)-LC/MS method. / Berkecz, R.; Tömösi, Ferenc; Körmöczi, Tímea; Szegedi, Viktor; Horváth, János; Janáky, T.

In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 149, 05.02.2018, p. 308-317.

Research output: Contribution to journalArticle

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