Composition and structure of microbial communities from stromatolites of Hamelin Pool in Shark Bay, Western Australia

Dominic Papineau, Jeffrey J. Walker, S. Mojzsis, Norman R. Pace

Research output: Contribution to journalArticle

146 Citations (Scopus)

Abstract

Stromatolites, organosedimentary structures formed by microbial activity, are found throughout the geological record and are important markers of biological history. More conspicuous in the past, stromatolites occur today in a few shallow marine environments, including Hamelin Pool in Shark Bay, Western Australia. Hamelin Pool stromatolites often have been considered contemporary analogs to ancient stromatolites, yet little is known about the microbial communities that build them. We used DNA-based molecular phylogenetic methods that do not require cultivation to study the microbial diversity of an irregular stromatolite and of the surface and interior of a domal stromatolite. To identify the constituents of the stromatolite communities, small subunit rRNA genes were amplified by PCR from community genomic DNA with universal primers, cloned, sequenced, and compared to known rRNA genes. The communities were highly diverse and novel. The average sequence identity of Hamelin Pool sequences compared to the > 200,000 known rRNA sequences was only ∼92%. Clone libraries were ∼90% bacterial and ∼10% archaeal, and eucaryotic rRNA genes were not detected in the libraries. The most abundant sequences were representative of novel proteobacteria (∼28%), planctomycetes (∼17%), and actinobacteria (∼14%). Sequences representative of cyanobacteria, long considered to dominate these communities, comprised

Original languageEnglish
Pages (from-to)4822-4832
Number of pages11
JournalApplied and Environmental Microbiology
Volume71
Issue number8
DOIs
Publication statusPublished - Aug 2005

Fingerprint

Sharks
stromatolite
Western Australia
shark
sharks
rRNA Genes
microbial communities
microbial community
ribosomal RNA
gene
DNA
Metagenomics
Proteobacteria
Actinobacteria
Cyanobacteria
geological record
Libraries
microbial activity
genes
cyanobacterium

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Composition and structure of microbial communities from stromatolites of Hamelin Pool in Shark Bay, Western Australia. / Papineau, Dominic; Walker, Jeffrey J.; Mojzsis, S.; Pace, Norman R.

In: Applied and Environmental Microbiology, Vol. 71, No. 8, 08.2005, p. 4822-4832.

Research output: Contribution to journalArticle

@article{83034f7457aa4e7d9160409ecc7cbe28,
title = "Composition and structure of microbial communities from stromatolites of Hamelin Pool in Shark Bay, Western Australia",
abstract = "Stromatolites, organosedimentary structures formed by microbial activity, are found throughout the geological record and are important markers of biological history. More conspicuous in the past, stromatolites occur today in a few shallow marine environments, including Hamelin Pool in Shark Bay, Western Australia. Hamelin Pool stromatolites often have been considered contemporary analogs to ancient stromatolites, yet little is known about the microbial communities that build them. We used DNA-based molecular phylogenetic methods that do not require cultivation to study the microbial diversity of an irregular stromatolite and of the surface and interior of a domal stromatolite. To identify the constituents of the stromatolite communities, small subunit rRNA genes were amplified by PCR from community genomic DNA with universal primers, cloned, sequenced, and compared to known rRNA genes. The communities were highly diverse and novel. The average sequence identity of Hamelin Pool sequences compared to the > 200,000 known rRNA sequences was only ∼92{\%}. Clone libraries were ∼90{\%} bacterial and ∼10{\%} archaeal, and eucaryotic rRNA genes were not detected in the libraries. The most abundant sequences were representative of novel proteobacteria (∼28{\%}), planctomycetes (∼17{\%}), and actinobacteria (∼14{\%}). Sequences representative of cyanobacteria, long considered to dominate these communities, comprised",
author = "Dominic Papineau and Walker, {Jeffrey J.} and S. Mojzsis and Pace, {Norman R.}",
year = "2005",
month = "8",
doi = "10.1128/AEM.71.8.4822-4832.2005",
language = "English",
volume = "71",
pages = "4822--4832",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "8",

}

TY - JOUR

T1 - Composition and structure of microbial communities from stromatolites of Hamelin Pool in Shark Bay, Western Australia

AU - Papineau, Dominic

AU - Walker, Jeffrey J.

AU - Mojzsis, S.

AU - Pace, Norman R.

PY - 2005/8

Y1 - 2005/8

N2 - Stromatolites, organosedimentary structures formed by microbial activity, are found throughout the geological record and are important markers of biological history. More conspicuous in the past, stromatolites occur today in a few shallow marine environments, including Hamelin Pool in Shark Bay, Western Australia. Hamelin Pool stromatolites often have been considered contemporary analogs to ancient stromatolites, yet little is known about the microbial communities that build them. We used DNA-based molecular phylogenetic methods that do not require cultivation to study the microbial diversity of an irregular stromatolite and of the surface and interior of a domal stromatolite. To identify the constituents of the stromatolite communities, small subunit rRNA genes were amplified by PCR from community genomic DNA with universal primers, cloned, sequenced, and compared to known rRNA genes. The communities were highly diverse and novel. The average sequence identity of Hamelin Pool sequences compared to the > 200,000 known rRNA sequences was only ∼92%. Clone libraries were ∼90% bacterial and ∼10% archaeal, and eucaryotic rRNA genes were not detected in the libraries. The most abundant sequences were representative of novel proteobacteria (∼28%), planctomycetes (∼17%), and actinobacteria (∼14%). Sequences representative of cyanobacteria, long considered to dominate these communities, comprised

AB - Stromatolites, organosedimentary structures formed by microbial activity, are found throughout the geological record and are important markers of biological history. More conspicuous in the past, stromatolites occur today in a few shallow marine environments, including Hamelin Pool in Shark Bay, Western Australia. Hamelin Pool stromatolites often have been considered contemporary analogs to ancient stromatolites, yet little is known about the microbial communities that build them. We used DNA-based molecular phylogenetic methods that do not require cultivation to study the microbial diversity of an irregular stromatolite and of the surface and interior of a domal stromatolite. To identify the constituents of the stromatolite communities, small subunit rRNA genes were amplified by PCR from community genomic DNA with universal primers, cloned, sequenced, and compared to known rRNA genes. The communities were highly diverse and novel. The average sequence identity of Hamelin Pool sequences compared to the > 200,000 known rRNA sequences was only ∼92%. Clone libraries were ∼90% bacterial and ∼10% archaeal, and eucaryotic rRNA genes were not detected in the libraries. The most abundant sequences were representative of novel proteobacteria (∼28%), planctomycetes (∼17%), and actinobacteria (∼14%). Sequences representative of cyanobacteria, long considered to dominate these communities, comprised

UR - http://www.scopus.com/inward/record.url?scp=23744506517&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=23744506517&partnerID=8YFLogxK

U2 - 10.1128/AEM.71.8.4822-4832.2005

DO - 10.1128/AEM.71.8.4822-4832.2005

M3 - Article

VL - 71

SP - 4822

EP - 4832

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 8

ER -