Complex spatial responses to cucumber mosaic virus infection in susceptible Cucurbita pepo cotyledons

Z. Havelda, A. J. Maule

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Cucumber mosaic virus infection of its susceptible host Cucurbita pepo results in a program of biochemical changes after virus infection. Applying a spatial analysis to expanding infected lesions, we investigated the relationship between the changes in enzyme activity and gene expression. Patterns of altered expression were seen that could not be detected by RNA gel blot analysis. For all the host genes studied, there was a downregulation (shutoff) of expression within the lesion. In addition, two distinct types of upregulation were observed. The expression of heat shock protein 70 (HSP70) and NADP+-dependent malic enzyme (NADP-ME) showed induction in apparently uninfected cells ahead of the infection. This response was more localized than the upregulation exhibited by catalase expression, which occurred throughout the uninfected regions of the tissue. The experiments showed that virus infection induced immediate and subsequent changes in gene expression by the host and that the infection has the potential to give advance signaling of the imminent infection.

Original languageEnglish
Pages (from-to)1975-1985
Number of pages11
JournalPlant Cell
Volume12
Issue number10
DOIs
Publication statusPublished - 2000

Fingerprint

Cucumovirus
Cucurbita
Cucumber mosaic virus
Cucurbita pepo
Cotyledon
Virus Diseases
Viruses
cotyledons
Gene expression
malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)
Up-Regulation
Infection
infection
Gene Expression
Enzyme Induction
Spatial Analysis
HSP70 Heat-Shock Proteins
Enzyme activity
NADP
Catalase

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Complex spatial responses to cucumber mosaic virus infection in susceptible Cucurbita pepo cotyledons. / Havelda, Z.; Maule, A. J.

In: Plant Cell, Vol. 12, No. 10, 2000, p. 1975-1985.

Research output: Contribution to journalArticle

@article{422c0f50caf741098263f312f2a05744,
title = "Complex spatial responses to cucumber mosaic virus infection in susceptible Cucurbita pepo cotyledons",
abstract = "Cucumber mosaic virus infection of its susceptible host Cucurbita pepo results in a program of biochemical changes after virus infection. Applying a spatial analysis to expanding infected lesions, we investigated the relationship between the changes in enzyme activity and gene expression. Patterns of altered expression were seen that could not be detected by RNA gel blot analysis. For all the host genes studied, there was a downregulation (shutoff) of expression within the lesion. In addition, two distinct types of upregulation were observed. The expression of heat shock protein 70 (HSP70) and NADP+-dependent malic enzyme (NADP-ME) showed induction in apparently uninfected cells ahead of the infection. This response was more localized than the upregulation exhibited by catalase expression, which occurred throughout the uninfected regions of the tissue. The experiments showed that virus infection induced immediate and subsequent changes in gene expression by the host and that the infection has the potential to give advance signaling of the imminent infection.",
author = "Z. Havelda and Maule, {A. J.}",
year = "2000",
doi = "10.1105/tpc.12.10.1975",
language = "English",
volume = "12",
pages = "1975--1985",
journal = "Plant Cell",
issn = "1040-4651",
publisher = "American Society of Plant Biologists",
number = "10",

}

TY - JOUR

T1 - Complex spatial responses to cucumber mosaic virus infection in susceptible Cucurbita pepo cotyledons

AU - Havelda, Z.

AU - Maule, A. J.

PY - 2000

Y1 - 2000

N2 - Cucumber mosaic virus infection of its susceptible host Cucurbita pepo results in a program of biochemical changes after virus infection. Applying a spatial analysis to expanding infected lesions, we investigated the relationship between the changes in enzyme activity and gene expression. Patterns of altered expression were seen that could not be detected by RNA gel blot analysis. For all the host genes studied, there was a downregulation (shutoff) of expression within the lesion. In addition, two distinct types of upregulation were observed. The expression of heat shock protein 70 (HSP70) and NADP+-dependent malic enzyme (NADP-ME) showed induction in apparently uninfected cells ahead of the infection. This response was more localized than the upregulation exhibited by catalase expression, which occurred throughout the uninfected regions of the tissue. The experiments showed that virus infection induced immediate and subsequent changes in gene expression by the host and that the infection has the potential to give advance signaling of the imminent infection.

AB - Cucumber mosaic virus infection of its susceptible host Cucurbita pepo results in a program of biochemical changes after virus infection. Applying a spatial analysis to expanding infected lesions, we investigated the relationship between the changes in enzyme activity and gene expression. Patterns of altered expression were seen that could not be detected by RNA gel blot analysis. For all the host genes studied, there was a downregulation (shutoff) of expression within the lesion. In addition, two distinct types of upregulation were observed. The expression of heat shock protein 70 (HSP70) and NADP+-dependent malic enzyme (NADP-ME) showed induction in apparently uninfected cells ahead of the infection. This response was more localized than the upregulation exhibited by catalase expression, which occurred throughout the uninfected regions of the tissue. The experiments showed that virus infection induced immediate and subsequent changes in gene expression by the host and that the infection has the potential to give advance signaling of the imminent infection.

UR - http://www.scopus.com/inward/record.url?scp=0033768589&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033768589&partnerID=8YFLogxK

U2 - 10.1105/tpc.12.10.1975

DO - 10.1105/tpc.12.10.1975

M3 - Article

C2 - 11041891

AN - SCOPUS:0033768589

VL - 12

SP - 1975

EP - 1985

JO - Plant Cell

JF - Plant Cell

SN - 1040-4651

IS - 10

ER -