Complex formation of yeast Rev1 with DNA polymerase η

Narottam Acharya, L. Haracska, Satya Prakash, Louise Prakash

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

In Saccharomyces cerevisiae, Rev1 functions in translesion DNA synthesis (TLS) together with polymerase ζ (Polζ), comprised of the Rev3 catalytic and Rev7 accessory subunits. Rev1 plays an indispensable structural role in promoting Polζ function, and deletion of the Rev1-C terminal region that is involved in physical interactions with Rev3 inactivates Polζ function in TLS. In humans, however, Rev1 has been shown to physically interact with the Y-family polymerases Polη Polι, and Polκ, and the Rev1 C terminus mediates these interactions. Since all the available genetic and biochemical evidence in yeast support the requirement of Rev1 as a structural element for Polζ and not for Polη, these observations have raised the possibility that in its structural role, Rev1 has diverged between yeast and humans. Here we show that although in yeast a stable Rev1-Polη complex can be formed, this complex formation involves the polymerase-associated domain of Rev1 and not the Rev1 C terminus as in humans. We also found that the DNA synthesis activity of Rev1 is enhanced in this complex. We discuss the implications of these and other observations for the possible divergence of Rev1's structural role between yeast and humans.

Original languageEnglish
Pages (from-to)8401-8408
Number of pages8
JournalMolecular and Cellular Biology
Volume27
Issue number23
DOIs
Publication statusPublished - Dec 2007

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DNA-Directed DNA Polymerase
Yeasts
DNA
Saccharomyces cerevisiae
Molecular Biology

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Complex formation of yeast Rev1 with DNA polymerase η. / Acharya, Narottam; Haracska, L.; Prakash, Satya; Prakash, Louise.

In: Molecular and Cellular Biology, Vol. 27, No. 23, 12.2007, p. 8401-8408.

Research output: Contribution to journalArticle

Acharya, Narottam ; Haracska, L. ; Prakash, Satya ; Prakash, Louise. / Complex formation of yeast Rev1 with DNA polymerase η. In: Molecular and Cellular Biology. 2007 ; Vol. 27, No. 23. pp. 8401-8408.
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