Complete sequence of raptor adenovirus 1 confirms the characteristic genome organization of siadenoviruses

Endre R. Kovács, M. Benkó

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Currently, the family Adenoviridae contains five genera, out of which Siadenovirus is one of the two least densely populated ones. A new member representing a new species in this genus has been detected in various birds of prey. The virus, named raptor adenovirus 1 (RAdV-1), could not be isolated, probably because no appropriate permissive cell-line was available. Partial genomic sequences, obtained by PCR and suggesting that the virus is a new siadenovirus species, have been published earlier. In the present paper, determination and analysis of the complete RAdV-1 genome are reported. This is the first complete genome sequence acquired from a non-isolated adenovirus (AdV). The sole source was a mixture of the internal organs of the diseased and dead birds. Until now, the genomic organization considered characteristic to siadenoviruses had been deduced from the detailed study of only two virus species, one of which originated from birds and the other from a frog. The present analysis of RAdV-1 confirmed the genus-specific genetic content and genomic features of siadenoviruses, and a putative novel gene was found as well. In general, AdVs and most of the AdV genera are thought to be strictly host specific. In the genus Siadenovirus, however, two virus species of rather divergent (avian and amphibian) host origin were present when the genus was found. Although by now the greatest number of known siadenoviruses infect birds, the original hosts of the genus remain unknown.

Original languageEnglish
Pages (from-to)1058-1065
Number of pages8
JournalInfection, Genetics and Evolution
Volume11
Issue number5
DOIs
Publication statusPublished - Jul 2011

Fingerprint

Raptor adenovirus A
Siadenovirus
Raptors
raptor
Adenoviridae
virus
genome
Genome
genomics
bird
Viruses
viruses
new species
Birds
birds
Bird Diseases
frog
amphibian
Amphibians
birds of prey

Keywords

  • Genome sequencing
  • PCR
  • Phylogeny
  • Siadenovirus

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Molecular Biology
  • Microbiology
  • Infectious Diseases
  • Microbiology (medical)

Cite this

Complete sequence of raptor adenovirus 1 confirms the characteristic genome organization of siadenoviruses. / Kovács, Endre R.; Benkó, M.

In: Infection, Genetics and Evolution, Vol. 11, No. 5, 07.2011, p. 1058-1065.

Research output: Contribution to journalArticle

@article{539187a40dc6462b9316ead68f89c6a9,
title = "Complete sequence of raptor adenovirus 1 confirms the characteristic genome organization of siadenoviruses",
abstract = "Currently, the family Adenoviridae contains five genera, out of which Siadenovirus is one of the two least densely populated ones. A new member representing a new species in this genus has been detected in various birds of prey. The virus, named raptor adenovirus 1 (RAdV-1), could not be isolated, probably because no appropriate permissive cell-line was available. Partial genomic sequences, obtained by PCR and suggesting that the virus is a new siadenovirus species, have been published earlier. In the present paper, determination and analysis of the complete RAdV-1 genome are reported. This is the first complete genome sequence acquired from a non-isolated adenovirus (AdV). The sole source was a mixture of the internal organs of the diseased and dead birds. Until now, the genomic organization considered characteristic to siadenoviruses had been deduced from the detailed study of only two virus species, one of which originated from birds and the other from a frog. The present analysis of RAdV-1 confirmed the genus-specific genetic content and genomic features of siadenoviruses, and a putative novel gene was found as well. In general, AdVs and most of the AdV genera are thought to be strictly host specific. In the genus Siadenovirus, however, two virus species of rather divergent (avian and amphibian) host origin were present when the genus was found. Although by now the greatest number of known siadenoviruses infect birds, the original hosts of the genus remain unknown.",
keywords = "Genome sequencing, PCR, Phylogeny, Siadenovirus",
author = "Kov{\'a}cs, {Endre R.} and M. Benk{\'o}",
year = "2011",
month = "7",
doi = "10.1016/j.meegid.2011.03.021",
language = "English",
volume = "11",
pages = "1058--1065",
journal = "Infection, Genetics and Evolution",
issn = "1567-1348",
publisher = "Elsevier",
number = "5",

}

TY - JOUR

T1 - Complete sequence of raptor adenovirus 1 confirms the characteristic genome organization of siadenoviruses

AU - Kovács, Endre R.

AU - Benkó, M.

PY - 2011/7

Y1 - 2011/7

N2 - Currently, the family Adenoviridae contains five genera, out of which Siadenovirus is one of the two least densely populated ones. A new member representing a new species in this genus has been detected in various birds of prey. The virus, named raptor adenovirus 1 (RAdV-1), could not be isolated, probably because no appropriate permissive cell-line was available. Partial genomic sequences, obtained by PCR and suggesting that the virus is a new siadenovirus species, have been published earlier. In the present paper, determination and analysis of the complete RAdV-1 genome are reported. This is the first complete genome sequence acquired from a non-isolated adenovirus (AdV). The sole source was a mixture of the internal organs of the diseased and dead birds. Until now, the genomic organization considered characteristic to siadenoviruses had been deduced from the detailed study of only two virus species, one of which originated from birds and the other from a frog. The present analysis of RAdV-1 confirmed the genus-specific genetic content and genomic features of siadenoviruses, and a putative novel gene was found as well. In general, AdVs and most of the AdV genera are thought to be strictly host specific. In the genus Siadenovirus, however, two virus species of rather divergent (avian and amphibian) host origin were present when the genus was found. Although by now the greatest number of known siadenoviruses infect birds, the original hosts of the genus remain unknown.

AB - Currently, the family Adenoviridae contains five genera, out of which Siadenovirus is one of the two least densely populated ones. A new member representing a new species in this genus has been detected in various birds of prey. The virus, named raptor adenovirus 1 (RAdV-1), could not be isolated, probably because no appropriate permissive cell-line was available. Partial genomic sequences, obtained by PCR and suggesting that the virus is a new siadenovirus species, have been published earlier. In the present paper, determination and analysis of the complete RAdV-1 genome are reported. This is the first complete genome sequence acquired from a non-isolated adenovirus (AdV). The sole source was a mixture of the internal organs of the diseased and dead birds. Until now, the genomic organization considered characteristic to siadenoviruses had been deduced from the detailed study of only two virus species, one of which originated from birds and the other from a frog. The present analysis of RAdV-1 confirmed the genus-specific genetic content and genomic features of siadenoviruses, and a putative novel gene was found as well. In general, AdVs and most of the AdV genera are thought to be strictly host specific. In the genus Siadenovirus, however, two virus species of rather divergent (avian and amphibian) host origin were present when the genus was found. Although by now the greatest number of known siadenoviruses infect birds, the original hosts of the genus remain unknown.

KW - Genome sequencing

KW - PCR

KW - Phylogeny

KW - Siadenovirus

UR - http://www.scopus.com/inward/record.url?scp=79956313173&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79956313173&partnerID=8YFLogxK

U2 - 10.1016/j.meegid.2011.03.021

DO - 10.1016/j.meegid.2011.03.021

M3 - Article

C2 - 21463713

AN - SCOPUS:79956313173

VL - 11

SP - 1058

EP - 1065

JO - Infection, Genetics and Evolution

JF - Infection, Genetics and Evolution

SN - 1567-1348

IS - 5

ER -