Complete lack of vitamin C intake generates pulmonary emphysema in senescence marker protein-30 knockout mice

Kengo Koike, Yoshitaka Kondo, Mitsuaki Sekiya, Yasunori Sato, Kazunori Tobino, Shin Iciro Iwakami, S. Goto, Kazuhisa Takahashi, Naoki Maruyama, Kuniaki Seyama, Akihito Ishigami

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Vitamin C (VC) is a potent antioxidant and plays an essential role in collagen synthesis. As we previously reported, senescence marker protein-30 (SMP30) knockout (KO) mice cannot synthesize VC due to the genetic disruption of gluconolactonase (i.e., SMP30). Here, we utilized SMP30 KO mice deprived of VC and found that VC depletion caused pulmonary emphysema due to oxidative stress and a decrease of collagen synthesis by the third month of age. We grew SMP30 KO mice and wild-type (WT) mice on VC-free chow and either VC water [VC(+)] or plain water [VC(-)] after weaning at 4 wk of age. Morphometric findings and reactive oxygen species (ROS) in the lungs were evaluated at 3 mo of age. No VC was detected in the lungs of SMP30 KO VC(-) mice, but their ROS increased 50.9% over that of the VC(+) group. Moreover, their collagen content in the lungs markedly decreased, and their collagen I mRNA decreased 82.2% compared with that of the WT VC(-) group. In the SMP30 KO VC(-) mice, emphysema developed [21.6% increase of mean linear intercepts (MLI) and 42.7% increase of destructive index compared with VC(+) groups], and the levels of sirtuin 1 (Sirt1) decreased 16.8%. However, VC intake increased the MLI 16.2% and thiobarbituric acid reactive substances 22.2% in WT mice, suggesting that an excess of VC can generate oxidative stress and may be harmful during this period of lung development. These results suggest that VC plays an important role in lung development through affecting oxidant-antioxidant balance and collagen synthesis.

Original languageEnglish
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume298
Issue number6
DOIs
Publication statusPublished - Jun 2010

Fingerprint

Pulmonary Emphysema
Knockout Mice
Ascorbic Acid
Proteins
Collagen
Lung
gluconolactonase
Reactive Oxygen Species
Oxidative Stress
Sirtuin 1
Antioxidants
Thiobarbituric Acid Reactive Substances
Water
Emphysema

Keywords

  • Chronic obstructive pulmonary disease
  • Collagen

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology
  • Physiology

Cite this

Complete lack of vitamin C intake generates pulmonary emphysema in senescence marker protein-30 knockout mice. / Koike, Kengo; Kondo, Yoshitaka; Sekiya, Mitsuaki; Sato, Yasunori; Tobino, Kazunori; Iwakami, Shin Iciro; Goto, S.; Takahashi, Kazuhisa; Maruyama, Naoki; Seyama, Kuniaki; Ishigami, Akihito.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 298, No. 6, 06.2010.

Research output: Contribution to journalArticle

Koike, Kengo ; Kondo, Yoshitaka ; Sekiya, Mitsuaki ; Sato, Yasunori ; Tobino, Kazunori ; Iwakami, Shin Iciro ; Goto, S. ; Takahashi, Kazuhisa ; Maruyama, Naoki ; Seyama, Kuniaki ; Ishigami, Akihito. / Complete lack of vitamin C intake generates pulmonary emphysema in senescence marker protein-30 knockout mice. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 2010 ; Vol. 298, No. 6.
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abstract = "Vitamin C (VC) is a potent antioxidant and plays an essential role in collagen synthesis. As we previously reported, senescence marker protein-30 (SMP30) knockout (KO) mice cannot synthesize VC due to the genetic disruption of gluconolactonase (i.e., SMP30). Here, we utilized SMP30 KO mice deprived of VC and found that VC depletion caused pulmonary emphysema due to oxidative stress and a decrease of collagen synthesis by the third month of age. We grew SMP30 KO mice and wild-type (WT) mice on VC-free chow and either VC water [VC(+)] or plain water [VC(-)] after weaning at 4 wk of age. Morphometric findings and reactive oxygen species (ROS) in the lungs were evaluated at 3 mo of age. No VC was detected in the lungs of SMP30 KO VC(-) mice, but their ROS increased 50.9{\%} over that of the VC(+) group. Moreover, their collagen content in the lungs markedly decreased, and their collagen I mRNA decreased 82.2{\%} compared with that of the WT VC(-) group. In the SMP30 KO VC(-) mice, emphysema developed [21.6{\%} increase of mean linear intercepts (MLI) and 42.7{\%} increase of destructive index compared with VC(+) groups], and the levels of sirtuin 1 (Sirt1) decreased 16.8{\%}. However, VC intake increased the MLI 16.2{\%} and thiobarbituric acid reactive substances 22.2{\%} in WT mice, suggesting that an excess of VC can generate oxidative stress and may be harmful during this period of lung development. These results suggest that VC plays an important role in lung development through affecting oxidant-antioxidant balance and collagen synthesis.",
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AU - Sekiya, Mitsuaki

AU - Sato, Yasunori

AU - Tobino, Kazunori

AU - Iwakami, Shin Iciro

AU - Goto, S.

AU - Takahashi, Kazuhisa

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AU - Seyama, Kuniaki

AU - Ishigami, Akihito

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AB - Vitamin C (VC) is a potent antioxidant and plays an essential role in collagen synthesis. As we previously reported, senescence marker protein-30 (SMP30) knockout (KO) mice cannot synthesize VC due to the genetic disruption of gluconolactonase (i.e., SMP30). Here, we utilized SMP30 KO mice deprived of VC and found that VC depletion caused pulmonary emphysema due to oxidative stress and a decrease of collagen synthesis by the third month of age. We grew SMP30 KO mice and wild-type (WT) mice on VC-free chow and either VC water [VC(+)] or plain water [VC(-)] after weaning at 4 wk of age. Morphometric findings and reactive oxygen species (ROS) in the lungs were evaluated at 3 mo of age. No VC was detected in the lungs of SMP30 KO VC(-) mice, but their ROS increased 50.9% over that of the VC(+) group. Moreover, their collagen content in the lungs markedly decreased, and their collagen I mRNA decreased 82.2% compared with that of the WT VC(-) group. In the SMP30 KO VC(-) mice, emphysema developed [21.6% increase of mean linear intercepts (MLI) and 42.7% increase of destructive index compared with VC(+) groups], and the levels of sirtuin 1 (Sirt1) decreased 16.8%. However, VC intake increased the MLI 16.2% and thiobarbituric acid reactive substances 22.2% in WT mice, suggesting that an excess of VC can generate oxidative stress and may be harmful during this period of lung development. These results suggest that VC plays an important role in lung development through affecting oxidant-antioxidant balance and collagen synthesis.

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