Complete genome sequence of Novosphingobium resinovorum SA1, a versatile xenobiotic-degrading bacterium capable of utilizing sulfanilic acid

Botond Hegedűs, P. Kós, Balázs Bálint, Gergely Maróti, Han Ming Gan, Katalin Perei, Gábor Rákhely

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Sulfanilic acid (4-aminobenzenesulfonic acid) is a sulfonated aromatic amine widely used in chemical industries for synthesis of various organic dyes and sulfa drugs. There are quite a few microbial co-cultures or single isolates capable of completely degrading this compound. Novosphingobium resinovorum SA1 was the first single bacterium which could utilize sulfanilic acid as its sole carbon, nitrogen and sulfur source. The strain has versatile catabolic routes for the bioconversion of numerous other aromatic compounds. Here, the complete genome sequence of the N. resinovorum SA1 strain is reported. The genome consists of a circular chromosome of 3.8 Mbp and four extrachromosomal elements between 67 and 1 759.8 kbp in size. Three alternative 3-ketoadipate pathways were identified on the plasmids. Sulfanilic acid is decomposed via a modified 3-ketoadipate pathway and the oxygenases involved form a phylogenetically separate branch on the tree. Sequence analysis of these elements might provide a genetic background for deeper insight into the versatile catabolic metabolism of various aromatic xenobiotics, including sulfanilic acid and its derivatives. Moreover, this is also a good model strain for understanding the role and evolution of multiple genetic elements within a single strain.

Original languageEnglish
Pages (from-to)76-80
Number of pages5
JournalJournal of Biotechnology
Volume241
DOIs
Publication statusPublished - Jan 10 2017

Fingerprint

Sulfanilic Acids
Xenobiotics
Bacteria
Genes
Genome
Acids
Chemical Industry
Synthetic Chemistry Techniques
Bioconversion
Oxygenases
Molecular Evolution
Aromatic compounds
Chromosomes
Chemical industry
Coculture Techniques
Sulfur
Metabolism
Amines
Sequence Analysis
Plasmids

Keywords

  • Aminobenzenesulfonate
  • Biodegradation of xenobiotics
  • Megaplasmid
  • Multiple extrachromosomal elements
  • Novosphingobium resinovorum SA1 genome

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Complete genome sequence of Novosphingobium resinovorum SA1, a versatile xenobiotic-degrading bacterium capable of utilizing sulfanilic acid. / Hegedűs, Botond; Kós, P.; Bálint, Balázs; Maróti, Gergely; Gan, Han Ming; Perei, Katalin; Rákhely, Gábor.

In: Journal of Biotechnology, Vol. 241, 10.01.2017, p. 76-80.

Research output: Contribution to journalArticle

Hegedűs, Botond ; Kós, P. ; Bálint, Balázs ; Maróti, Gergely ; Gan, Han Ming ; Perei, Katalin ; Rákhely, Gábor. / Complete genome sequence of Novosphingobium resinovorum SA1, a versatile xenobiotic-degrading bacterium capable of utilizing sulfanilic acid. In: Journal of Biotechnology. 2017 ; Vol. 241. pp. 76-80.
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