Complete DNA sequence and analysis of a mitochondrial plasmid in the mycoparasitic Trichoderma harzianum strain T95

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9 Citations (Scopus)

Abstract

A circular plasmid called pThr1, with a monomer size of 2.6 kb, was identified in the mitochondria of a Trichoderma harzianum isolate. Hybridization studies using cloned plasmids revealed no DNA sequence similarity between the plasmid and the mitochondrial genome of the isolate. The complete sequence of the plasmid was determined, and the sequence analysis revealed that it contained a single long open reading frame of 1818 bp. Sequence comparisons indicated that the derived amino acid sequence of the ORF exhibited similarity to the reverse transcriptases of the circular Mauriceville and Varkud retroplasmids of Neurospora spp. and the linear pFOXC2 and pFOXC3 retroplasmids of Fusarium oxysporum strains. In the regions of homology all of the seven conserved amino acid blocks characteristic of RTs could be found.

Original languageEnglish
Pages (from-to)148-152
Number of pages5
JournalPlasmid
Volume47
Issue number2
DOIs
Publication statusPublished - 2002

Fingerprint

Trichoderma
DNA Sequence Analysis
Plasmids
Open Reading Frames
Neurospora
Mitochondrial Genome
RNA-Directed DNA Polymerase
Fusarium
Sequence Analysis
Amino Acid Sequence
Mitochondria
Amino Acids

Keywords

  • DNA sequence
  • Mitochondrial plasmid
  • Reverse transcriptase
  • Trichoderma harzianum

ASJC Scopus subject areas

  • Molecular Biology

Cite this

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title = "Complete DNA sequence and analysis of a mitochondrial plasmid in the mycoparasitic Trichoderma harzianum strain T95",
abstract = "A circular plasmid called pThr1, with a monomer size of 2.6 kb, was identified in the mitochondria of a Trichoderma harzianum isolate. Hybridization studies using cloned plasmids revealed no DNA sequence similarity between the plasmid and the mitochondrial genome of the isolate. The complete sequence of the plasmid was determined, and the sequence analysis revealed that it contained a single long open reading frame of 1818 bp. Sequence comparisons indicated that the derived amino acid sequence of the ORF exhibited similarity to the reverse transcriptases of the circular Mauriceville and Varkud retroplasmids of Neurospora spp. and the linear pFOXC2 and pFOXC3 retroplasmids of Fusarium oxysporum strains. In the regions of homology all of the seven conserved amino acid blocks characteristic of RTs could be found.",
keywords = "DNA sequence, Mitochondrial plasmid, Reverse transcriptase, Trichoderma harzianum",
author = "Zsuzsanna Antal and L. Manczinger and L. Kredics and F. Kevei and E. Nagy",
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journal = "Plasmid",
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T1 - Complete DNA sequence and analysis of a mitochondrial plasmid in the mycoparasitic Trichoderma harzianum strain T95

AU - Antal, Zsuzsanna

AU - Manczinger, L.

AU - Kredics, L.

AU - Kevei, F.

AU - Nagy, E.

PY - 2002

Y1 - 2002

N2 - A circular plasmid called pThr1, with a monomer size of 2.6 kb, was identified in the mitochondria of a Trichoderma harzianum isolate. Hybridization studies using cloned plasmids revealed no DNA sequence similarity between the plasmid and the mitochondrial genome of the isolate. The complete sequence of the plasmid was determined, and the sequence analysis revealed that it contained a single long open reading frame of 1818 bp. Sequence comparisons indicated that the derived amino acid sequence of the ORF exhibited similarity to the reverse transcriptases of the circular Mauriceville and Varkud retroplasmids of Neurospora spp. and the linear pFOXC2 and pFOXC3 retroplasmids of Fusarium oxysporum strains. In the regions of homology all of the seven conserved amino acid blocks characteristic of RTs could be found.

AB - A circular plasmid called pThr1, with a monomer size of 2.6 kb, was identified in the mitochondria of a Trichoderma harzianum isolate. Hybridization studies using cloned plasmids revealed no DNA sequence similarity between the plasmid and the mitochondrial genome of the isolate. The complete sequence of the plasmid was determined, and the sequence analysis revealed that it contained a single long open reading frame of 1818 bp. Sequence comparisons indicated that the derived amino acid sequence of the ORF exhibited similarity to the reverse transcriptases of the circular Mauriceville and Varkud retroplasmids of Neurospora spp. and the linear pFOXC2 and pFOXC3 retroplasmids of Fusarium oxysporum strains. In the regions of homology all of the seven conserved amino acid blocks characteristic of RTs could be found.

KW - DNA sequence

KW - Mitochondrial plasmid

KW - Reverse transcriptase

KW - Trichoderma harzianum

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