Complementation of a yeast cell cycle mutant by an alfalfa cDNA encoding a protein kinase homologous to p34cdc2

Heribert Hirt, Anikó Páy, J. Györgyey, László Bakó, Kinga Németh, László Bögre, Rudolf J. Schweyen, Erwin Heberle-Bors, D. Dudits

Research output: Contribution to journalArticle

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Abstract

The cdc2 protein kinase plays a central role in control of the eukaryotic cell cycle of animals and yeasts. We have isolated a cDNA clone (cdc2Ms) from alfalfa (Medicago sativa L.) that is homologous to the yeast cdc2/CDC28 genes. The encoded protein is 64% identical to the yeast and mammalian counterparts and shows all the prominent structural features known from these organisms. Antibody raised against a 16-amino acid synthetic peptide with crossreactivity against p34 proteins recognized a 34-kilodalton protein in extracts of alfalfa cells. When transferred into a fission yeast, the plant cdc2 homolog can complement a temperature-sensitive cdc2 mutant. Northern analysis revealed higher transcript levels in shoots and suspension cultures than in roots. In addition to the dominant transcript of 1.4 kilobases detected in the poly(A)+ fraction, 2.5- and 1.2-kilobase transcripts were detected in total RNA preparations from shoots or somatic embryos. Suspension cultures that were induced to form somatic embryos by an auxin (2,4-dichlorophenoxyacetic acid) showed fluctuations in transcription pattern during the induction period and embryogenesis.

Original languageEnglish
Pages (from-to)1636-1640
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number5
Publication statusPublished - 1991

Fingerprint

Medicago sativa
Protein Kinases
Cell Cycle
Complementary DNA
Yeasts
Suspensions
Embryonic Structures
CDC2 Protein Kinase
2,4-Dichlorophenoxyacetic Acid
Proteins
Poly A
Indoleacetic Acids
Schizosaccharomyces
Eukaryotic Cells
Cell Cycle Checkpoints
Cell Extracts
Embryonic Development
Clone Cells
RNA
Amino Acids

Keywords

  • 2,4-dichlorophenoxyacetic acid
  • Medicago saliva L
  • Plant cell culture
  • Plant cell cycle
  • Somatic embryogenesis

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Complementation of a yeast cell cycle mutant by an alfalfa cDNA encoding a protein kinase homologous to p34cdc2 . / Hirt, Heribert; Páy, Anikó; Györgyey, J.; Bakó, László; Németh, Kinga; Bögre, László; Schweyen, Rudolf J.; Heberle-Bors, Erwin; Dudits, D.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, No. 5, 1991, p. 1636-1640.

Research output: Contribution to journalArticle

Hirt, Heribert ; Páy, Anikó ; Györgyey, J. ; Bakó, László ; Németh, Kinga ; Bögre, László ; Schweyen, Rudolf J. ; Heberle-Bors, Erwin ; Dudits, D. / Complementation of a yeast cell cycle mutant by an alfalfa cDNA encoding a protein kinase homologous to p34cdc2 In: Proceedings of the National Academy of Sciences of the United States of America. 1991 ; Vol. 88, No. 5. pp. 1636-1640.
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