Complementation by detached parts of GGCC-specific DNA methyltransferases

G. Pósfai, Sun C. Kim, L. Szilák, Attila Kovács, Pál Venetianer

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Individually inactive N- and C-terminal fragments of the m5C-methyltransferase M.BspRI can complement each other resulting in specific, in vivo methylation of the DNA. This was shown by cloning the coding regions for N- and C-terminal parts of the enzyme in compatible plasmids and co-transforming them into E.coli cells. The enzyme could be detached at several different sites, producing either non-overlapping or partially overlapping fragments capable of complementation. Reconstitution of the active methyltransferase from inactive fragments was demonstrated in vitro, as well. Another GGCC-specific methyltransferase, M.BsuRI, showed a similar complementation phenomenon. Moreover, interspecies complementation was observed between appropriate fragments of the two closely related enzymes M.BspRI and M.BsuRI. Fragments of structurally and functionally more different methyltransferases were unable to complement each other.

Original languageEnglish
Pages (from-to)4843-4847
Number of pages5
JournalNucleic Acids Research
Volume19
Issue number18
Publication statusPublished - Sep 25 1991

Fingerprint

Complementation
Fragment
DNA
Enzymes
Methyltransferases
Methylation
Cloning
DNA Methylation
Complement
Escherichia coli
Organism Cloning
Plasmids
Escherichia Coli
Overlapping
Coding
Cytosine 5-methyltransferase
Cell

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

Complementation by detached parts of GGCC-specific DNA methyltransferases. / Pósfai, G.; Kim, Sun C.; Szilák, L.; Kovács, Attila; Venetianer, Pál.

In: Nucleic Acids Research, Vol. 19, No. 18, 25.09.1991, p. 4843-4847.

Research output: Contribution to journalArticle

Pósfai, G, Kim, SC, Szilák, L, Kovács, A & Venetianer, P 1991, 'Complementation by detached parts of GGCC-specific DNA methyltransferases', Nucleic Acids Research, vol. 19, no. 18, pp. 4843-4847.
Pósfai, G. ; Kim, Sun C. ; Szilák, L. ; Kovács, Attila ; Venetianer, Pál. / Complementation by detached parts of GGCC-specific DNA methyltransferases. In: Nucleic Acids Research. 1991 ; Vol. 19, No. 18. pp. 4843-4847.
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