Complement protein C1q induces maturation of human dendritic cells

Eszter Csomor, Z. Bajtay, Noémi Sándor, Katalin Kristóf, Gérard J. Arlaud, Steffen Thiel, A. Erdei

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Maturation of dendritic cells (DCs) is known to be induced by several stimuli, including microbial products, inflammatory cytokines and immobilized IgG, as demonstrated recently. Since immune complexes formed in vivo also contain C1q, moreover apoptotic cells and several pathogens fix C1q in the absence of antibodies, we undertook to investigate whether this complement protein has an impact on various functions of human DCs. Maturation of monocyte-derived immature DCs (imMDCs) cultured on immobilized C1q was followed by monitoring expression of CD80, CD83, CD86, MHCII and CCR7. The functional activity of the cells was assessed by measuring cytokine secretion and their ability to activate allogeneic T lymphocytes. Cytokine production by T cells co-cultured with C1q-matured DCs was also investigated. C1q, but not the structurally related mannose-binding lectin was found to bind to imMDC in a dose-dependent manner and induced NF-κB translocation to the nucleus. Immobilized C1q induced maturation of MDCs and enhanced secretion of IL-12 and TNF-α, moreover, elevated their T-cell stimulating capacity. As IFN-γ levels were increased in supernatants of MDC-T cell co-cultures, our data suggest that C1q-induced DC maturation generates a Th1-type response. Interestingly, IL-10 levels were elevated by C1q-treated MDCs but not in the supernatant of their co-cultures with allogeneic T cells. Taken together, these results indicate that C1q-opsonized antigens may play a role in the induction and regulation of immune response. Moreover our data are relevant in view of the role of C1q in removal of apoptotic cells and the association between C1q-deficiency and autoimmunity.

Original languageEnglish
Pages (from-to)3389-3397
Number of pages9
JournalMolecular Immunology
Volume44
Issue number13
DOIs
Publication statusPublished - Jul 2007

Fingerprint

Complement C1q
Dendritic Cells
Complement System Proteins
T-Lymphocytes
Cytokines
Coculture Techniques
Mannose-Binding Lectin
Interleukin-12
Antigen-Antibody Complex
Autoimmunity
Interleukin-10
Monocytes
Cell Culture Techniques
Immunoglobulin G
Antigens
Antibodies

Keywords

  • Complement C1q
  • Dendritic cell
  • NF-κB translocation
  • Th1-response

ASJC Scopus subject areas

  • Molecular Biology
  • Immunology

Cite this

Complement protein C1q induces maturation of human dendritic cells. / Csomor, Eszter; Bajtay, Z.; Sándor, Noémi; Kristóf, Katalin; Arlaud, Gérard J.; Thiel, Steffen; Erdei, A.

In: Molecular Immunology, Vol. 44, No. 13, 07.2007, p. 3389-3397.

Research output: Contribution to journalArticle

Csomor, Eszter ; Bajtay, Z. ; Sándor, Noémi ; Kristóf, Katalin ; Arlaud, Gérard J. ; Thiel, Steffen ; Erdei, A. / Complement protein C1q induces maturation of human dendritic cells. In: Molecular Immunology. 2007 ; Vol. 44, No. 13. pp. 3389-3397.
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