Complement peptides and mast cell triggering

A. Erdei, Israel Pecht

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Mucosal type mast cells have been earlier shown to be unresponsive to the so called 'peptidergic' stimulus provided by cationic agents, such as anaphylatoxins, neuropeptides or polyamines. We studied the relationship between mast cells' secretory response to stimulation via their type 1 Fcε receptors (FcεRI) and that provided by C5a and C3a fragments of the complement system, in the rat mucosal-type mast cell line RBL-2H3. Our results shown here reveal a novel function of C3a, its inhibitory capacity on IgE-mediated triggering of mucosal mast cells. This activity of C3a is most probably mediated by its interaction with the β-chain of FcεRI. While connective tissue type mast cells are known to be activated by micromolar concentrations of the complement peptides C3a and C5a, the amount of C3a necessary for the inhibition of antigen-induced degranulation of mucosal cells in our assays is in the nanomolar range. Interestingly, the other anaphylatoxic peptide C5a, which is known to be much more effective in several biological assays, did not show any activity in the same test-system.

Original languageEnglish
Pages (from-to)109-112
Number of pages4
JournalImmunology Letters
Volume54
Issue number2-3
DOIs
Publication statusPublished - Dec 2 1996

Fingerprint

Mast Cells
Complement C3a
Peptides
Complement C5a
Anaphylatoxins
Connective Tissue Cells
Cell Degranulation
Fc Receptors
Polyamines
Neuropeptides
Biological Assay
Immunoglobulin E
Antigens
Cell Line

Keywords

  • C3a
  • C5a
  • FcεRI
  • IgE-mediated triggering
  • inhibition
  • mucosal mast cells
  • RBL-2H3 line

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Complement peptides and mast cell triggering. / Erdei, A.; Pecht, Israel.

In: Immunology Letters, Vol. 54, No. 2-3, 02.12.1996, p. 109-112.

Research output: Contribution to journalArticle

Erdei, A. ; Pecht, Israel. / Complement peptides and mast cell triggering. In: Immunology Letters. 1996 ; Vol. 54, No. 2-3. pp. 109-112.
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