Complement peptide C3a inhibits IgEmediated triggering of rat mucosal mast cells

Anna Erdei, Sergey Andreev, Israel Pecht

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The relationship between mast cells' secretory response to stimulation via their type 1 Fcε receptors (FcεRI) and that provided by the C3a fragment of the complement system was Investigated in the rat mucosal-type mast cell line RBL-2H3. These cells are known to be unresponsive to the so-called 'peptidergic' stimulus provided by cationlc agents, such as anaphylatoxlns, neuropeptides or polyamines. We now observed that C3a effectively inhibits the FceRI clustering Induced secretion of RBL-2H3 cells. This inhibition is dose-dependent and takes place at a C3a concentration range of 0.4-12.5 nM, I.e. at least three orders of magnitude lower than those where this anaphylatoxln exerts its secretory stimulus to 'serosal' mast cells. In order to identify where C3a interferes In the FceRI coupling cascade, we have studied its effect on the cells' protein phosphorylatlon pattern, hydrolysis of phosphatldyl Inositides, transient rise in free cytosollc Ca2+ Ion concentration and Ca2+ uptake. All these processes were found to be inhibited by a similar C3a concentration range.

Original languageEnglish
Pages (from-to)1433-1439
Number of pages7
JournalInternational Immunology
Volume7
Issue number9
DOIs
Publication statusPublished - Sep 1 1995

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Keywords

  • C3a
  • FcεRI
  • Mast cell
  • RBL-2H3 line
  • Signal tarnsduction
  • Triggering

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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