Complement-fixing antibodies to human cytomegalovirus induced early nuclear antigens in mononucleosis

L. Gergely, Judith Czeglédy, L. Váczi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Early nuclear antigen (CMNA) induced by human cytomegalovirus (HCMV) was extracted from infected human embryonic fibroblast cells and purified by ds DNA Sephadex chromatography. The purified antigen was added to acid-fixed preparations from human embryonic fibroblasts and these in vitro converted nuclei were exposed to human sera to estimate the antibodies to CMNA by anti-complement immunofluorescence staining. Serial serum samples were obtained from eight patients suffering from acute HCMV infection (mononucleosis), from nine patients with Epstein-Barr virus (EBV) mononucleosis, and from 20 healthy persons who had been shown to possess antibodies to HCMV late antigens. Acute HCMV infection is characterized by the presence of anti-CMNA antibodies at high titer (1:16-1:32) together with the elevated level of IgG antibodies to anti-HCMV-late antigens. During convalescence the anti-CMNA titer decreased to a lower level which was maintained for long period. The anti-CMNA antibodies were also regularly detected in sera of persons possessing antibodies to HCMV late antigens but without any sign of acute HCMV infection. It is concluded that antibodies to CMNA are not transitory and their presence even at high titer (1:16-1:32) in a serum sample cannot be taken as a presumptive evidence of acute virus infection. This method seems to be valuable for measuring antibodies to nuclear DNA-binding antigens induced by other viruses.

Original languageEnglish
Pages (from-to)99-108
Number of pages10
JournalMedical Microbiology and Immunology
Volume170
Issue number2
DOIs
Publication statusPublished - Dec 1981

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Antibodies
Cytomegalovirus
Cytomegalovirus Infections
Antigens
Anti-Idiotypic Antibodies
Serum
Fibroblasts
cytomegalovirus immediate-early proteins
Nuclear Antigens
DNA
Virus Diseases
Human Herpesvirus 4
Fluorescent Antibody Technique
Chromatography
Immunoglobulin G
Staining and Labeling
Viruses
Acids

ASJC Scopus subject areas

  • Microbiology (medical)
  • Immunology and Allergy
  • Immunology

Cite this

Complement-fixing antibodies to human cytomegalovirus induced early nuclear antigens in mononucleosis. / Gergely, L.; Czeglédy, Judith; Váczi, L.

In: Medical Microbiology and Immunology, Vol. 170, No. 2, 12.1981, p. 99-108.

Research output: Contribution to journalArticle

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