Complement consumption, hemolysis and thromboxane secretion following injection of hemoglobin-containing liposomes in rats

J. Szebeni, N. Wassef, H. H. Spielberg, G. Matyas, R. O. Cliff, A. S. Rudolph, C. R. Alving

Research output: Contribution to journalArticle

Abstract

Liposome-encapsulated hemoglobin (LEH) was administered intravenously to rats and plasma samples were taken at intervals from 10 up to 180 min after injection. The samples displayed increased free Hb and thromboxane B2 levels, decreased complement hemolytic titers and increased lytic activity on glucose-containing liposomes in an in vitro assay. In vitro studies showed that traces of phospholipase A2 activity, present in hemoglobin (Hb), were amplified 20 to 300-fold when co-encapsulated with Hb in liposomes. There is a possibility that a causal relationship exists between lysolecithin production and transient hemodynamic and hematological effects of LEH in rats.

Original languageEnglish
JournalArtificial Cells, Blood Substitutes, and Immobilization Biotechnology
Volume22
Issue number5
Publication statusPublished - Nov 1994

Fingerprint

Liposomes
Thromboxanes
Hemoglobin
Hemolysis
Rats
Hemoglobins
Injections
Thromboxane B2
Lysophosphatidylcholines
Phospholipases A2
Hemodynamics
Glucose
Assays
Complement System Proteins
Plasmas
In Vitro Techniques

ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering
  • Biomaterials
  • Hematology

Cite this

Complement consumption, hemolysis and thromboxane secretion following injection of hemoglobin-containing liposomes in rats. / Szebeni, J.; Wassef, N.; Spielberg, H. H.; Matyas, G.; Cliff, R. O.; Rudolph, A. S.; Alving, C. R.

In: Artificial Cells, Blood Substitutes, and Immobilization Biotechnology, Vol. 22, No. 5, 11.1994.

Research output: Contribution to journalArticle

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AU - Spielberg, H. H.

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AU - Cliff, R. O.

AU - Rudolph, A. S.

AU - Alving, C. R.

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