Complement component 1q subcomponent binding protein in the brain of the rat

János Barna, Diána Dimén, Gina Puska, Dávid Kovács, Vivien Csikós, Szilvia Oláh, Edina B. Udvari, Gabriella Pál, A. Dobolyi

Research output: Contribution to journalArticle

Abstract

Complement component 1q subcomponent binding protein (C1qbp) is a multifunctional protein involved in immune response, energy homeostasis of cells as a plasma membrane receptor, and a nuclear, cytoplasmic or mitochondrial protein. Recent reports suggested its neuronal function, too, possibly in axon maintenance, synaptic function, and neuroplasticity. Therefore, we addressed to identify C1qbp in the rat brain using in situ hybridization histochemistry and immunolabelling at light and electron microscopic level. C1qbp has a topographical distribution in the brain established by the same pattern of C1qbp mRNA-expressing and protein-containing neurons with the highest abundance in the cerebral cortex, anterodorsal thalamic nucleus, hypothalamic paraventricular (PVN) and arcuate nuclei, spinal trigeminal nucleus. Double labelling of C1qbp with the neuronal marker NeuN, with the astrocyte marker S100, and the microglia marker Iba1 demonstrated the presence of C1qbp in neurons but not in glial cells in the normal brain, while C1qbp appeared in microglia following their activation induced by focal ischemic lesion. Only restricted neurons expressed C1qbp, for example, in the PVN, magnocellular neurons selectively contained C1qbp. Further double labelling by using the mitochondria marker Idh3a antibody suggested the mitochondrial localization of C1qbp in the brain, confirmed by correlated light and electron microscopy at 3 different brain regions. Post-embedding immunoelectron microscopy also suggested uneven C1qbp content of mitochondria in different brain areas but also heterogeneity within single neurons. These data suggest a specific function of C1qbp in the brain related to mitochondria, such as the regulation of local energy supply in neuronal cells.

Original languageEnglish
Article number4597
JournalScientific reports
Volume9
Issue number1
DOIs
Publication statusPublished - Dec 1 2019

Fingerprint

Complement C1q
Carrier Proteins
Brain
Neurons
Mitochondria
Microglia
Spinal Trigeminal Nucleus
Anterior Thalamic Nuclei
Light
Arcuate Nucleus of Hypothalamus
Neuronal Plasticity
Immunoelectron Microscopy
Paraventricular Hypothalamic Nucleus
Mitochondrial Proteins
Nuclear Proteins
Neuroglia
Astrocytes
Cerebral Cortex
In Situ Hybridization

ASJC Scopus subject areas

  • General

Cite this

Complement component 1q subcomponent binding protein in the brain of the rat. / Barna, János; Dimén, Diána; Puska, Gina; Kovács, Dávid; Csikós, Vivien; Oláh, Szilvia; Udvari, Edina B.; Pál, Gabriella; Dobolyi, A.

In: Scientific reports, Vol. 9, No. 1, 4597, 01.12.2019.

Research output: Contribution to journalArticle

Barna, J, Dimén, D, Puska, G, Kovács, D, Csikós, V, Oláh, S, Udvari, EB, Pál, G & Dobolyi, A 2019, 'Complement component 1q subcomponent binding protein in the brain of the rat', Scientific reports, vol. 9, no. 1, 4597. https://doi.org/10.1038/s41598-019-40788-z
Barna J, Dimén D, Puska G, Kovács D, Csikós V, Oláh S et al. Complement component 1q subcomponent binding protein in the brain of the rat. Scientific reports. 2019 Dec 1;9(1). 4597. https://doi.org/10.1038/s41598-019-40788-z
Barna, János ; Dimén, Diána ; Puska, Gina ; Kovács, Dávid ; Csikós, Vivien ; Oláh, Szilvia ; Udvari, Edina B. ; Pál, Gabriella ; Dobolyi, A. / Complement component 1q subcomponent binding protein in the brain of the rat. In: Scientific reports. 2019 ; Vol. 9, No. 1.
@article{09ccbfc797334cebb5925716dc33191d,
title = "Complement component 1q subcomponent binding protein in the brain of the rat",
abstract = "Complement component 1q subcomponent binding protein (C1qbp) is a multifunctional protein involved in immune response, energy homeostasis of cells as a plasma membrane receptor, and a nuclear, cytoplasmic or mitochondrial protein. Recent reports suggested its neuronal function, too, possibly in axon maintenance, synaptic function, and neuroplasticity. Therefore, we addressed to identify C1qbp in the rat brain using in situ hybridization histochemistry and immunolabelling at light and electron microscopic level. C1qbp has a topographical distribution in the brain established by the same pattern of C1qbp mRNA-expressing and protein-containing neurons with the highest abundance in the cerebral cortex, anterodorsal thalamic nucleus, hypothalamic paraventricular (PVN) and arcuate nuclei, spinal trigeminal nucleus. Double labelling of C1qbp with the neuronal marker NeuN, with the astrocyte marker S100, and the microglia marker Iba1 demonstrated the presence of C1qbp in neurons but not in glial cells in the normal brain, while C1qbp appeared in microglia following their activation induced by focal ischemic lesion. Only restricted neurons expressed C1qbp, for example, in the PVN, magnocellular neurons selectively contained C1qbp. Further double labelling by using the mitochondria marker Idh3a antibody suggested the mitochondrial localization of C1qbp in the brain, confirmed by correlated light and electron microscopy at 3 different brain regions. Post-embedding immunoelectron microscopy also suggested uneven C1qbp content of mitochondria in different brain areas but also heterogeneity within single neurons. These data suggest a specific function of C1qbp in the brain related to mitochondria, such as the regulation of local energy supply in neuronal cells.",
author = "J{\'a}nos Barna and Di{\'a}na Dim{\'e}n and Gina Puska and D{\'a}vid Kov{\'a}cs and Vivien Csik{\'o}s and Szilvia Ol{\'a}h and Udvari, {Edina B.} and Gabriella P{\'a}l and A. Dobolyi",
year = "2019",
month = "12",
day = "1",
doi = "10.1038/s41598-019-40788-z",
language = "English",
volume = "9",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Complement component 1q subcomponent binding protein in the brain of the rat

AU - Barna, János

AU - Dimén, Diána

AU - Puska, Gina

AU - Kovács, Dávid

AU - Csikós, Vivien

AU - Oláh, Szilvia

AU - Udvari, Edina B.

AU - Pál, Gabriella

AU - Dobolyi, A.

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Complement component 1q subcomponent binding protein (C1qbp) is a multifunctional protein involved in immune response, energy homeostasis of cells as a plasma membrane receptor, and a nuclear, cytoplasmic or mitochondrial protein. Recent reports suggested its neuronal function, too, possibly in axon maintenance, synaptic function, and neuroplasticity. Therefore, we addressed to identify C1qbp in the rat brain using in situ hybridization histochemistry and immunolabelling at light and electron microscopic level. C1qbp has a topographical distribution in the brain established by the same pattern of C1qbp mRNA-expressing and protein-containing neurons with the highest abundance in the cerebral cortex, anterodorsal thalamic nucleus, hypothalamic paraventricular (PVN) and arcuate nuclei, spinal trigeminal nucleus. Double labelling of C1qbp with the neuronal marker NeuN, with the astrocyte marker S100, and the microglia marker Iba1 demonstrated the presence of C1qbp in neurons but not in glial cells in the normal brain, while C1qbp appeared in microglia following their activation induced by focal ischemic lesion. Only restricted neurons expressed C1qbp, for example, in the PVN, magnocellular neurons selectively contained C1qbp. Further double labelling by using the mitochondria marker Idh3a antibody suggested the mitochondrial localization of C1qbp in the brain, confirmed by correlated light and electron microscopy at 3 different brain regions. Post-embedding immunoelectron microscopy also suggested uneven C1qbp content of mitochondria in different brain areas but also heterogeneity within single neurons. These data suggest a specific function of C1qbp in the brain related to mitochondria, such as the regulation of local energy supply in neuronal cells.

AB - Complement component 1q subcomponent binding protein (C1qbp) is a multifunctional protein involved in immune response, energy homeostasis of cells as a plasma membrane receptor, and a nuclear, cytoplasmic or mitochondrial protein. Recent reports suggested its neuronal function, too, possibly in axon maintenance, synaptic function, and neuroplasticity. Therefore, we addressed to identify C1qbp in the rat brain using in situ hybridization histochemistry and immunolabelling at light and electron microscopic level. C1qbp has a topographical distribution in the brain established by the same pattern of C1qbp mRNA-expressing and protein-containing neurons with the highest abundance in the cerebral cortex, anterodorsal thalamic nucleus, hypothalamic paraventricular (PVN) and arcuate nuclei, spinal trigeminal nucleus. Double labelling of C1qbp with the neuronal marker NeuN, with the astrocyte marker S100, and the microglia marker Iba1 demonstrated the presence of C1qbp in neurons but not in glial cells in the normal brain, while C1qbp appeared in microglia following their activation induced by focal ischemic lesion. Only restricted neurons expressed C1qbp, for example, in the PVN, magnocellular neurons selectively contained C1qbp. Further double labelling by using the mitochondria marker Idh3a antibody suggested the mitochondrial localization of C1qbp in the brain, confirmed by correlated light and electron microscopy at 3 different brain regions. Post-embedding immunoelectron microscopy also suggested uneven C1qbp content of mitochondria in different brain areas but also heterogeneity within single neurons. These data suggest a specific function of C1qbp in the brain related to mitochondria, such as the regulation of local energy supply in neuronal cells.

UR - http://www.scopus.com/inward/record.url?scp=85062941203&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85062941203&partnerID=8YFLogxK

U2 - 10.1038/s41598-019-40788-z

DO - 10.1038/s41598-019-40788-z

M3 - Article

C2 - 30872665

AN - SCOPUS:85062941203

VL - 9

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 4597

ER -